中草药2026，Vol.57Issue(16)：6233-6241,9.DOI:10.7501/j.issn.0253-2670.2026.16.003
旋覆花中2个新倍半萜二聚体成分及体外抗肝癌活性
Two new sesquiterpenoid dimers from Inula japonica and their in vitro anti-hepatocellular carcinoma activity
摘要
Abstract
Objective To investigate the chemical constituents from the inflorescences of Inula japonica and their in vitro anti-hepatocellular carcinoma activity.Methods The compounds were isolated and purified by multiple chromatographic techniques..Their structures were identified by HRESIMS,NMR,IR,UV spectroscopic methods,and the absolute configurations of new compounds were determined by calculated NMR and ECD.The inhibitory effects of the compounds on the proliferation of HepG2 cells were evaluated by MTT assay and colony formation assay.Results Six sesquiterpenoid dimers were isolated from the ethyl acetate fraction of the 90％ethanol extract of I.japonica,among which compounds 1(inujaponolide T)and 3(inujaponolide U)were identified as new compounds.The bioactivity screening results showed that compounds 1-6 exhibited potent cytotoxicity against HepG2 cells,with IC50 values ranging from 2.61 to 13.94 μmol/L.Furthermore,compounds 1 and 3 inhibited the cell viability and reduced the colony formation of HepG2 cells in a dose-dependent manner.Conclusion Compounds 1 and 3 are new sesquiterpenoid dimers with anti-hepatocellular carcinoma activity,and may serve as potential antitumor lead compounds for further in-depth investigation.关键词
旋覆花属/旋覆花/倍半萜二聚体/旋覆花倍半萜二聚体内酯T/旋覆花倍半萜二聚体内酯U/抗肝癌活性Key words
Inula Thunb./Inula japonica Thunb./sesquiterpenoid dimers/inujaponolides T/inujaponolides U/anti-hepatocellular carcinoma activity分类
医药卫生引用本文复制引用
于航,胡栋宝,董伟,刘贵有,于金正,赵永雪,黄明思,靳海香,苏璇,李千姿,陈明轩..旋覆花中2个新倍半萜二聚体成分及体外抗肝癌活性[J].中草药,2026,57(16):6233-6241,9.基金项目
云南省地方高校基础研究专项面上项目(202301AO070134) （202301AO070134）
云南省教育厅科学研究基金青年项目(2024J0889) （2024J0889）
省级大学生创新创业训练计划(S202511390017,S202611390065) （S202511390017,S202611390065）
