中草药2026，Vol.57Issue(16)：6320-6328,9.DOI:10.7501/j.issn.0253-2670.2026.16.009
葛根素-壳聚糖修饰β-环糊精鼻用超分子凝胶的制备及抗偏头痛药效评价
Preparation and anti-migraine pharmacodynamic evaluation of puerarin-loaded chitosan-modified β-cyclodextrin nasal supramolecular gel
摘要
Abstract
Objective To develop a puerarin-loaded supramolecular gel based on chitosan-modified β-cyclodextrin(Pur@CS-β-CD Gel),optimize its formulation,and evaluate its potential for nasal delivery and anti-migraine efficacy.Methods The Pur@CS-β-CD Gel formulation was optimized using single-factor experiments and Box-Behnken design-response surface methodology(BBD-RSM).The optimized Pur@CS-β-CD Gel was characterized for its microstructure,pH value,stability,and in vitro release profile.A chronic migraine rat model was established.The therapeutic efficacy was assessed through behavioral indicators and by measuring the levels of calcitonin gene-related peptide(CGRP),5-hydroxytryptamine(5-HT),and interleukin-1 β(IL-1β)in brain tissue.Results The optimal formulation consisted of CS-β-CD and sodium carboxymethylcellulose at a mass ratio of 1∶9,a total polymer concentration of 2.6％,and 40 mg of puerarin.The resulting Pur@CS-β-CD Gel exhibited a three-dimensional porous structure,a pH of 6.5,good stability,and a biphasic in vitro release profile characterized by an initial rapid release(82.75％within 4 h)followed by sustained release,with no obvious nasal-mucosal irritation.Pharmacodynamic studies demonstrated that Pur@CS-β-CD Gel significantly improved migraine-related behavioral performance in rats,reduced CGRP and IL-1 β levels,and increased 5-HT content in the brain.Conclusion The Pur@CS-β-CD Gel demonstrates a feasible preparation process,favorable properties for nasal administration,and effective anti-migraine activity,providing an experimental basis for the development of novel nasal delivery systems and migraine therapies.关键词
葛根素/超分子凝胶/工艺优化/鼻腔给药/慢性偏头痛/药效评价Key words
puerarin/supramolecular gel/process optimization/nasal administration/chronic migraine/pharmacodynamic evaluation分类
医药卫生引用本文复制引用
宗时宇,王苗,马鑫宇,刘烁,任佳,程云龙,王春柳..葛根素-壳聚糖修饰β-环糊精鼻用超分子凝胶的制备及抗偏头痛药效评价[J].中草药,2026,57(16):6320-6328,9.基金项目
陕西省重点研发计划一般项目(2024SF-YBXM-530) （2024SF-YBXM-530）
陕西省科学技术协会青年人才托举计划(20250355) （20250355）
陕西省中医药管理局科研项目(SZY-KJCYC-2025-ZY-009) （SZY-KJCYC-2025-ZY-009）
陕西省中医药研究院(陕西省中医医院)院级"苗圃培育计划"(2025-18) （陕西省中医医院）
