针刺研究2026，Vol.51Issue(8)：1013-1021,9.DOI:10.13702/j.1000-0607.20250698
电针"心俞"通过抑制铁死亡途径改善大鼠心肌缺血的研究
Electroacupuncture at"Xinshu"(BL15)improves myocardial ischemia by inhibiting the ferroptosis pathway in rats
摘要
Abstract
Objective To explore the role of the ferroptosis pathway of myocardial cells in electroacupuncture(EA)at"Xinshu"(BL15)-induced improvement of myocardial ischemia(MI)in rats.Methods A total of 18 male SD rats were randomly divided into control,model and EA groups,with 6 rats in each group.The MI model was established by subcutaneous injection of isoproterenol(ISO,60 mg/kg)for consecutive 2 d.The rats of the control group received the same volume of 0.9%sodium chloride solution for 2 d.EA was applied to bilateral BL15 for 30 min,once a day for 3 d.The electrocardiogram(ECG)of the standard limb lead II was recorded before and after modeling,and after the intervention using a multi-channel physiological recorder.Histopathological changes of the myocardial tissue were observed by hematoxylin-eosin staining(HE staining),and the ultrastructural changes of the myocardial cell mitochondria were observed under a transmission electron microscope(TEM).The contents of divalent iron ions(Fe2+),malondialdehyde(MDA),and glutathione(GSH),as well as the activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-PX)in the myocardial tissue were detected using biochemical methods.The expression levels of ferritin heavy chain 1(FTH1),nuclear factor E2-related factor 2(Nrf2),solute carrier family 7 member 11(SLC7A11),glutathione peroxidase 4(GPX4)and acyl-CoA synthetase long-chain family member 4(ACSL4)mRNAs and proteins in the myocardial tissue were detected using real-time fluorescence quantitative PCR and Western blot,separately.Results Compared with the control group,the model group had a significant increase in the ECG ST segment after modeling and a decrease after interventions,content of Fe2+,and the expression levels of ACSL4 mRNA and protein(P<0.01,P<0.05),and a considerable decrease in the content of GSH,activity of SOD and GSH-PX,and the expression levels of FTH1,Nrf2,SLC7A11,and GPX4 mRNAs and proteins(P<0.01).In contrast to the model group,modeling induced abnormalities of ECG-ST segment,content of Fe2+,and the expression levels of ACSL4 mRNA and protein and the decrease of the content of GSH,activity of SOD and GSH-PX,and the expression levels of FTH1,Nrf2,SLC7A11,and GPX4 mRNAs and proteins were all reversed by EA(P<0.01,P<0.05).Histopathological and ultrastructural observations showed a reduction in the number of cardiomyocytes,with loose arrangement,extracellular inflammatory cell infiltration,vacuolated myocardial fibers and increased extracellular space,and a decrease in the mitochondrial volume,with an increase of density and breakage of crista in the model group,which was relatively milder in the injury degree of cardiomyocytes and their mitochondria,etc.in the EA group.Conclusion EA at BL15 can alleviate ischemic myocardium injury in rats with MI,which may be achieved by inhibiting the ferroptosis signaling pathway in the myocardial cells.关键词
心肌缺血/电针/心俞/铁死亡途径Key words
Myocardial ischemia/Electroacupuncture/Xinshu(BL15)/Ferroptosis pathway引用本文复制引用
迟小稳,董亚琴,许金森,郑淑霞,曹小敏,万隆..电针"心俞"通过抑制铁死亡途径改善大鼠心肌缺血的研究[J].针刺研究,2026,51(8):1013-1021,9.基金项目
国家自然科学基金青年基金项目(No.81403490) （No.81403490）
福建省自然科学基金项目(No.2022J01857) （No.2022J01857）
福建中医药大学2024年度针灸学科开放课题项目(No.XZJ2024005) （No.XZJ2024005）
福建省中医药重点学科建设项目"针灸工程学" （）
