中国临床医学2026，Vol.33Issue(4)：615-621,7.DOI:10.12025/j.issn.1008-6358.2026.20260382
脐血Treg细胞FOXP3 mRNA、IL-10和TGF-β对婴儿早期特应性皮炎的预测价值
Predictive value of FOXP3 mRNA,IL-10 and TGF-β in cord blood Tregs for early-onset atopic dermatitis in infants
摘要
Abstract
Objective To explore the predictive value of forkhead box P3(FOXP3)mRNA expression in cord blood derived regulatory T cells(Tregs)and the associated cytokines interleukin-10(IL-10)and transforming growth factor-β(TGF-β)for early-onset atopic dermatitis(AD)in infants.Methods The healthy full-term newborns delivered vaginally at Shanghai Fifth People's Hospital from September 2023 to April 2024 were enrolled.Umbilical vein blood was collected at birth to measure FOXP3 mRNA expression by RT-PCR and IL-10 and TGF-β concentrations by ELISA.Follow-up was performed at 6 weeks,3 months,and 6 months postnatally via telephone or outpatient visits to assess the occurrence of AD in infants.Based on the occurrence of AD at 6 months of age,the infants were divided into an AD group and a control group.The indicators related to mothers and infants were compared between the two groups.Logistic regression analysis was used to identify risk factors for the development of AD in infants within 6 months after birth,and a predictive model was constructed.The performance of the model in predicting early-onset AD was evaluated using receiver operating characteristic(ROC)curve analysis.Results A total of 59 infants completed 6-month follow-up,with 17(28.8%)diagnosed with AD.No significant differences were observed in gestational age,sex,birth weight,presence of siblings,smoking expossure,maternal dietary preferences(egg,milk,or seafood intake),maternal age and education level,or cord blood IL-10 levels between two groups.There were significant differences in season of birth,family history of atopic disease,feeding method,and cord blood levels of FOXP3 mRNA and TGF-β between two groups(P＜0.05).Logistic regression analysis showed low levels of cord blood FOXP3 mRNA and TGF-β,and family history of atopic disease were risk factors for early-onset AD in infants(P＜0.05).A predictive model was constructed using risk factors.The area under the curve of the combined model was 0.821(95%CI 0.680-0.962),with a sensitivity of 76.5%and a specificity of 85.7%.Conclusion Reduced cord blood FOXP3 mRNA and TGF-β level,and a family history of atopic diseases could be used to predict early-onset AD in infants.关键词
婴儿/早期特应性皮炎/脐血/叉头盒转录因子P3/白细胞介素10/转化生长因子βKey words
infant/early-onset atopic dermatitis/cord blood/forkhead box P3/interleukin-10/transforming growth factor-β分类
医药卫生引用本文复制引用
黄晓晴,赵诸慧,沈奕,李英华,张振花..脐血Treg细胞FOXP3 mRNA、IL-10和TGF-β对婴儿早期特应性皮炎的预测价值[J].中国临床医学,2026,33(4):615-621,7.基金项目
上海市闵行区自然科学研究项目(2023MHZ062).Supported by Natural Science Research Project of Minhang District,Shanghai(2023MHZ062). （2023MHZ062）
