摘要
Abstract
The forensic DNA database constructed based on capillary electrophoresis(CE)technology is an important foundation of modern forensic science and plays an irreplaceable role in public security and social governance in China.With the growing application needs,the limitations of CE-based DNA databases have become increasingly evident,while massively parallel sequencing(MPS)technology provides an opportunity for DNA database iteration.This paper briefly reviews the history of DNA database construction in China during the CE era,explores the valuable experience gained from attempts to apply MPS technology in DNA database exploratory construction,and focuses on key directions for future MPS-based DNA database upgrading in China.These include achieving compatibility of CE/MPS-STR allele length typing,screening for more new core STR loci,establishing quality control systems for MPS-STR typing detection,deepening complex kinship searches in DNA databases,strengthening Y-STR paternal kinship searches,and advancing the construction of forensic mitochondrial DNA databases.The establishment of an MPS-based DNA database is not merely a technical iteration but also a multidimensional reconstruction and improvement of database structure,data comparison,and quality control.It is expected that these findings will better serve forensic practice in China and contribute Chinese experience to the international construction of forensic DNA databases based on MPS.关键词
法医遗传学/毛细管电泳测序/大规模平行测序/DNA数据库Key words
forensic genetics/capillary electrophoresis sequencing/massively parallel sequencing(MPS)/DNA database分类
社会科学
