中国饲料Issue(15)：35-44,10.DOI:10.15906/j.cnki.cn11-2975/s.2025030020-07
响应面优化黑果腺肋花楸总三萜的提取及其抑菌活性和稳定性研究
Optimization of extraction of total triterpenoids from Aronia melanocarpa using response surface methodology:antibacterial activity and stability studies
摘要
Abstract
The experiment used Aronia melanocarpa as the raw material and employed ultrasonic-assisted ethanol extraction to extract total triterpenes.The extraction process was optimized using response surface methodology(RSM)with the goal of maximizing the total triterpene content in Aronia melanocarpa.The antibacterial activity,antioxidant capacity,hemolytic activity,cytotoxicity,and stability of the extract were systematically evaluated.The results indicated that the optimal extraction conditions for total triterpenes were an alcohol concentration of 61%,a solid-to-liquid ratio of 1:36(g/mL),and an extraction time of 80 min,yielding a total triterpene content of 4.442 mg/g.The extract demonstrated significant antimicrobial activity against Escherichia coli and Staphylococcus aureus,with minimum bactericidal concentration(MBC)of 8 mg/mL and 1.25 mg/mL,respectively.Additionally,at a concentration of 0.25 mg/mL,the extract exhibited a free radical scavenging rate of up to 99%,showing excellent antioxidant capacity.Furthermore,the hemolysis rate of the extract was below 10%at concentrations 10～1.25 mg/mL,and cytotoxicity tests revealed over 95%cell viability after 12 hours,indicating good biosafety and cell selectivity.However,the extract showed some sensitivity in environments containing salt ions and enzyme solutions,with the MBC increasing 2～8 times.This research provides a theoretical basis for ennancing the deep-processing level and the added value of Aronia melanocarpa products,as well as its applications in the fields of food additives and animal feed additive.关键词
黑果腺肋花楸/总三萜/响应面法/抑菌活性Key words
Aronia melanocarpa/total triterpenes/response surface methodology/antibacterial activity分类
农业科技引用本文复制引用
杨添泽,徐彤,张继一,刘畅,董帅,刘叶,江涛,唐玉娇..响应面优化黑果腺肋花楸总三萜的提取及其抑菌活性和稳定性研究[J].中国饲料,2026,(15):35-44,10.基金项目
2026年吉林省高教科研重点自筹课题(JGJX26C301) （JGJX26C301）
2024年国家级大学生创新创业训练计划项目(202413606005) （202413606005）
