摘要
Abstract
Objective To preliminarily explore the microbiome information of apheresis platelets from healthy blood do-nors in Nanjing,Jiangsu,and to lay the groundwork for further studies on the microbial community composition and diversity in this region.Methods Thirteen fresh apheresis platelet samples(5 mL each)were collected from voluntary blood donors at the Nanjing Red Cross Blood Center.After centrifugation and removal of the supernatant,DNA was extracted from the pellet and libraries were constructed.High-throughput sequencing was performed on the Illumina NovaSeq X Plus platform,followed by in-depth bioinformatics analysis.Metagenomic analysis software,including fastp,Bowtie 2,MEGAHIT,Meta-GeneMark,DIAMOND,and Micro_NR,were used to analyze microbial abundance in the apheresis platelet samples.Re-sults Using Krona for visualization of species annotation and metagenomic sequencing analysis,after removing the human genome background,a total of 71 phyla,54 classes,102 orders,181 families,421 genera,and 1 340 species of microbes were identified.Starting from relative abundance tables at different taxonomic levels,at the family level,the top 10 most a-bundant families were:Flavobacteriaceae;Moraxellaceae;Rhizobiaceae;Sphingomonadaceae;Comamonadaceae;Cau-lobacteraceae;Burkholderiaceae;Acidimicrobiaceae;Streptomycetaceae;Streptosporangiaceae.At the genus level,the top 10 most abundant genera were:Wenyingzhuangia,Acinetobacter,Aquabacterium,Rhizobium,Brevundimonas,Novosphingobi-um,Astrithrvirus,Unclassified Rhizobiaceae,Acidiferrimicrobium,and Limnobacter.Conclusion Next-generation sequen-cing(NGS)was used to comprehensively annotate the metagenome of apheresis platelets from voluntary blood donors in Nan-jing,Jiangsu,enabling rapid acquisition of extensive microbial data.Exploratory analysis of the blood microbiome in healthy populations provides valuable guidance for developing context-specific measures to prevent platelet bacterial contamination,thereby enhancing blood safety.关键词
单采血小板/微生物组/宏基因Key words
apheresis platelets/microbiome/metagenomics分类
医药卫生
