中国现代医学杂志2026，Vol.36Issue(16)：36-44,9.DOI:10.3969/j.issn.1005-8982.2026.16.006
慢性盆腔痛湿热瘀结证大鼠模型的构建与验证评价
Establishment and validation of a rat model of chronic pelvic pain with dampness-heat stasis syndrome
摘要
Abstract
Objective To establish a multidimensional evaluation system integrating traditional Chinese medicine(TCM)macroscopic syndrome manifestations and modern medical microscopic indicators,to scientifically validate the efficacy,feasibility,and applicability of a rat model of chronic pelvic pain with dampness-heat stasis syndrome induced by phenol mucilage chemical injury,and therefore to provide a standardized experimental platform for investigating the pathogenesis and developing therapeutic agents for this disease.Methods Twenty female Sprague-Dawley(SD)rats were randomly divided into a blank group,a sham-operated group and a model group using a random number table.The blank group was raised routinely.The sham-operated group only received laparotomy and a layered closure of the abdomen without chemical injury.The model group was established by intrauterine injection of 25%phenol mucilage to induce chemical damage.After modeling,a systematic evaluation was performed from four aspects:(1)Macroscopic manifestations:general conditions and body weight changes of rats were observed,damp-heat stasis syndrome scores were assessed using a standardized scale,and fecal water content was measured.(2)Behavioral tests:mechanical pain threshold was determined using an electronic von Frey anesthesiometer to quantify pelvic pain severity.(3)Local pathological evaluation:pelvic tissue adhesion,masses and effusion were anatomically observed,and uterine tissues were stained with hematoxylin-eosin(HE)for histopathological analysis.(4)Serological and hemorheological assays:serum levels of inflammatory factors[prostaglandin E2(PGE2),vascular endothelial growth factor(VEGF),interleukin-1 alpha(IL-1α),and interleukin-2(IL-2)]were detected by enzyme-linked immunosorbent assay(ELISA),while whole blood viscosity,plasma viscosity and other indices were measured using a fully automatic hemorheology analyzer.Results Compared with the blank group and sham-operated group,rats in the model group exhibited typical and stable pathological characteristics of damp-heat stasis syndrome and chronic pelvic pain.Macroscopically,the model rats presented listlessness,anorexia,inactivity,withered hair,perianal dampness,loose and sticky stools,significantly slower body weight gain,and notably higher damp-heat stasis syndrome scores(P<0.05).Behavioral tests showed a markedly decreased mechanical pain threshold(P<0.05).Pathological anatomy revealed extensive adhesion and thickening of pelvic tissues.HE staining of uterine tissues demonstrated chronic inflammatory cell infiltration,fibrous tissue hyperplasia and destruction of the luminal structure.Serological tests indicated significantly upregulated levels of pro-inflammatory factors IL-1α and PGE2,elevated fibrosis-related factor VEGF,and reduced immunoregulatory factor IL-2(P<0.05).Hemorheological indices showed abnormally increased whole blood viscosity and plasma viscosity(P<0.05),as well as decreased red blood cell aggregation index(P<0.05).Conclusion The chemical injury method with phenol mucilage can successfully establish a rat model featuring both damp-heat stasis syndrome and pathological changes of chronic pelvic pain.This model shows favorable feasibility and applicability,and may serve as an ideal experimental tool for integrated traditional Chinese and Western medicine research on chronic pelvic pain.关键词
慢性盆腔痛/湿热瘀结证/苯酚胶浆/动物模型/多维度评价Key words
chronic pelvic pain/dampness-heat stasis syndrome/phenol mucilage/animal model/multidimensional evaluation分类
医药卫生引用本文复制引用
谭钰蒙,张锁,赵思宇,王广龙,关金花,郝海霞..慢性盆腔痛湿热瘀结证大鼠模型的构建与验证评价[J].中国现代医学杂志,2026,36(16):36-44,9.基金项目
国家自然科学基金(82560903) （82560903）
