中国瓜菜2026，Vol.39Issue(8)：31-36,6.DOI:10.16861/j.cnki.zggc.2025.0743
外源茉莉酸甲酯对干旱胁迫下甘蓝生理特性及BoPRX基因表达的影响
Effects of exogenous methyl jasmonate on physiological characteristics and BoPRX gene expression of cabbage under drought stress
摘要
Abstract
To investigate the alleviation effect and relguatory mechanism of methyl jasmonate(MeJA)on cabbage seed-lings under drought stress,the seedling of Qiu Tian 2 cabbage was used as experimental materials.Three treatments were established:CK(normal growth conditions),T1(15%PEG8000-induced drought stress),and T2(drought stress with 15%PEG8000 was applied after spraying with 25 μmol·L-1 MeJA).Chlorophyll content,relative electrical conductivity(REC),malondialdehyde(MDA)content,antioxidant enzyme activity,and the expression patterns of peroxidase genes(PRX)were measured at 0,3,6,12,and 24 h post-treatment.The results indicated that compared with CK,chlorophyll content in T1-treated seedlings decreased,while REC and MDA content increased.Activity of superoxide dismutase(SOD),peroxidase(POD),and catalase(CAT)(except treatment for 24 h)progressively increased over time under stress conditions.However,after T2 treatment,chlorophyll content,as well as the activity of SOD,POD,and CAT was higher than that in T1,while the REC and MDA content were reduced compared to the T1 treatment.The expression of the five BoPRX genes rapidly and were up-regulated expression in the early stages of both T1 and T2 treatments but were down-regulated expression after 12 or 24 h.Notably,except for BoPRX113,the expression level of other four BoPRX genes under T2 treatment were lower than those under T1 treatment.In conclusion,exogenous MeJA can alleviate the damage of drought stress to cabbage seedlings and enhance their drought resistance by maintaining chlorophyll content,reducing cell membrane damage,regulating the activity of antioxidant enzymes,and modulating the expression of BoPRX genes.The findings of this study provide a theoretical basis for drought-resistant cultivation of cabbage.关键词
结球甘蓝/MeJA/干旱胁迫/生理效应/PRX基因Key words
Cabbage/MeJA/Drought stress/Physiological effect/PRXgene分类
农业科技引用本文复制引用
李美慧,周甜甜,宋泽政,闫帅,孔依,王玉书..外源茉莉酸甲酯对干旱胁迫下甘蓝生理特性及BoPRX基因表达的影响[J].中国瓜菜,2026,39(8):31-36,6.基金项目
黑龙江省自然科学基金(PL2024C036) （PL2024C036）
黑龙江省普通高等学校青年创新人才培养计划(UNPYSCT-2017155) （UNPYSCT-2017155）
国家级大学生创新创业项目(202510232150X) （202510232150X）
