中国医科大学学报2026，Vol.55Issue(8)：680-686,7.DOI:10.12007/j.issn.0258-4646.2026.08.002
PTBP1通过激活FGFBP1/FGFR1/ERK信号通路降低宫颈癌细胞放疗敏感性
PTBP1 reduces radiosensitivity of cervical cancer cells by activating the FGFBP1/FGFR1/ERK signaling pathway
摘要
Abstract
Objective To investigate the effect of polypyrimidine tract-binding protein 1(PTBP1)on the radiosensitivity of cervical cancer cells and to elucidate the underlying mechanism involving fibroblast growth factor binding protein 1(FGFBP1).Methods SiHa cells were divided into ov-NC,ov-PTBP1,ov-PTBP1+sh-NC and ov-PTBP1+sh-FGFBP1 groups.Radioresistant SiHa(SiHa-RR)cells were divided into sh-NC,sh-PTBP1,sh-PTBP1+ov-NC and sh-PTBP1+ov-FGFBP1.Stable transfection was achieved using lentiviral vectors.Cell radiosensitivity was evaluated using CCK-8 assays,flow cytometry,and Transwell assays.RT-qPCR was used to measure PTBP1 and FGFBP1 mRNA expression levels.Protein expression levels of PTBP1,FGFBP1,p-FGFR1,and phosphorylated ERK1/2(p-ERK1/2)were detected by Western blotting.Results Overexpression of PTBP1 in irradiated SiHa cells increased cell viability,migration,and invasion,decreased apoptosis,and upregulated the expression of FGFBP1,p-FGFR1,and p-ERK1/2.In contrast,PTBP1 knockdown in irradiated SiHa-RR cells reduced cell viability,migration,and invasion,increased apoptosis,and downregulated the expres-sion of FGFBP1,p-FGFR1,and p-ERK1/2.Furthermore,FGFBP1 knockdown in PTBP1-overexpressing SiHa cells reversed these effects,while FGFBP1 overexpression in PTBP1-silenced SiHa-RR cells restored cell viability,migration,and invasion,decreased apoptosis,and increased the expression of p-FGFR1 and p-ERK1/2.Conclusion PTBP1 reduces the radiosensitivity of cervical cancer cells by upregu-lating FGFBP1 expression,potentially by activating the FGFR1/ERK signaling pathway.关键词
宫颈癌/多嘧啶束结合蛋白1/成纤维细胞生长因子结合蛋白1/放疗敏感性/FGFR1/ERK信号通路Key words
cervical cancer/polypyrimidine tract-binding protein 1/fibroblast growth factor binding protein 1/radiotherapy sensitivity/FGFR1/ERK signaling pathway分类
医药卫生引用本文复制引用
李枫,包超恩,刘胤良,曹晓辉,朱晓娅,刘兵,翟福山..PTBP1通过激活FGFBP1/FGFR1/ERK信号通路降低宫颈癌细胞放疗敏感性[J].中国医科大学学报,2026,55(8):680-686,7.基金项目
河北省医学科学研究课题计划(20230092) （20230092）
