中国医科大学学报2026，Vol.55Issue(8)：700-706,7.DOI:10.12007/j.issn.0258-4646.2026.08.005
miR-4677-3p通过靶向调控KLF7影响结直肠癌细胞增殖、凋亡和侵袭
miR-4677-3p regulates the proliferation,apoptosis and invasion of colorectal cancer cells by targeting and modulating KLF7
摘要
Abstract
Objective To explore the effects of miR-4677-3p on the proliferation,apoptosis,and invasion of colorectal cancer(CRC)cells by targeting KLF7.Methods Real-time PCR and Western blotting were used to detect miR-4677-3p and KLF7 expression in CRC tissues and cells.HCT116 cells were assigned into miR-NC,miR-4677-3p mimics,si-NC,si-KLF7,miR-4677-3p mimics+pcDNA3.1,and miR-4677-3p mimics+pcDNA-KLF7 groups.Cell cloning experiments,flow cytometry,and Transwell assay were used to detect cell proliferation,apoptosis,and invasion,respectively.Western blotting was used to test proliferation,apoptosis,and invasion-related pro-teins.The nude mouse transplant tumor experiment was used to observe the growth of the transplant tumor.Real-time PCR was performed to detect miR-4677-3p in tumor tissue.Immunohistochemistry was performed to detect the proteins related to tumor tissue proliferation,apoptosis,and invasion.Results In the CRC tissues and CRC cells HCT116,SW480,and HT29,miR-4677-3p was downregulated and KLF7 was upregulated(P<0.05).The miR-4677-3p mimic group showed a decrease in the number of cell clones formed,the number of cell invasions,KLF7,Ki-67,MMP-9,and CLDN1,and an increase in the apoptosis rate and cleaved caspase-3 when compared to the miR-NC group(P<0.05).For the si-NC group,the si-KLF7 group showed a decrease in the number of cell clones formed,the number of cell invasions,Ki-67,MMP-9,and CLDN1,and an increase in the apoptosis rate and cleaved caspase-3 when compared to the si-NC group(P<0.05).The miR-4677-3p mimics+pcDNA-KLF7 group showed an increase in the number of cell clones formed,the number of cell invasions,KLF7,Ki-67,MMP-9,and CLDN1,and a decrease in the apoptosis rate and cleaved caspase-3 compared to the miR-4677-3p mimics+pcDNA3.1 group(P<0.05).The starBase website prediction and dual-luciferase assay reports indicated a targeted relationship between miR-4677-3p and KLF7.In the miR-NC group,the miR-4677-3p mimics group showed a decrease in regard to the tumor volume and mass,an increase in miR-4677-3p and cleaved caspase-3 levels,and a decrease in KLF7,Ki-67,MMP-9,and CLDN1(P<0.05).Conclusion miR-4677-3p can inhibit CRC cell proliferation,invasion,and promote apoptosis by targeting KLF7.关键词
miR-4677-3p/KLF7/结直肠癌/增殖/凋亡/侵袭Key words
miR-4677-3p/KLF7/colorectal cancer/proliferation/apoptosis/invasion分类
医药卫生引用本文复制引用
康嘉敏,高紫玉,刘妍慧,乌日罕,刘彩霞,邢智伟..miR-4677-3p通过靶向调控KLF7影响结直肠癌细胞增殖、凋亡和侵袭[J].中国医科大学学报,2026,55(8):700-706,7.基金项目
内蒙古自治区"草原英才"工程创新创业团队项目(ZY20241207) （ZY20241207）
