针灸推拿医学（英文版）2026，Vol.24Issue(4)：330-338,9.DOI:10.1007/s11726-026-1583-8
温和灸足三里对再生障碍性贫血小鼠造血功能及Notch信号通路相关因子表达的影响
Effects of mild moxibustion at Zusanli(ST36)on hematopoietic function and expression of Notch signaling pathway-related factors in aplastic anemia mice
摘要
Abstract
Objective:To observe the effects of mild moxibustion at Zusanli(ST36)on hematopoietic function and expression of Notch signaling pathway key factors in aplastic anemia(AA)mice,thereby exploring the potential mechanism of mild moxibustion in AA treatment. Methods:Ten mice were randomly selected as the normal control group from 28 male C57BL/6J mice,and the other 18 were injected with cyclophosphamide(CTX)and cyclosporine(Cs)to prepare the AA model.Successfully validated model mice were randomly divided into a model group and a moxibustion group,with 8 mice in each group.Mice in the normal control group were fed routinely,with same fixation and grasping performed as in the moxibustion group.After modeling,mice in the moxibustion group received mild moxibustion at unilateral Zusanli(ST36),once daily for 15 min each time.Bilateral Zusanli(ST36)were used alternately with consecutive moxibustion for 60 d.The proliferation of bone marrow cells and the structure of bone marrow hematopoietic tissue were observed in each group.The proportion of CD34+cells in bone marrow was detected by flow cytometry.Detection of protein and mRNA expression levels of Jagged canonical Notch ligand 1(Jagged1),Notch receptor 1(Notch1),delta-like ligand 4(DLL4),and Hes family BHLH transcription factor 1(Hes1)in bone marrow CD34+cells was performed by Western blotting and real-time fluorescence quantitative polymerase chain reaction. Results:Compared with the normal control group,hematopoietic cell numbers and hematopoietic tissue proportions in mouse bone marrow in the model group were significantly decreased(P<0.01).Compared with the model group,hematopoietic cell numbers and hematopoietic tissue proportions in mouse bone marrow in the moxibustion group were significantly increased(P<0.01,P<0.05).Compared with the normal control group,the CD34+cell proportion in mouse bone marrow in the model group was decreased significantly(P<0.01).Compared with the model group,CD34+cell proportion in mouse bone marrow in the moxibustion group was increased significantly(P<0.01).Compared with the normal control group,the protein and mRNA expression levels of Notch1,Jagged1,DLL4,and Hes1 in mouse bone marrow CD34+cells in the model group were significantly decreased(P<0.01).Compared with the model group,the above protein and mRNA expression levels in mouse bone marrow CD34+cells in the moxibustion group were significantly increased(P<0.01). Conclusion:Mild moxibustion at Zusanli(ST36)improves hematopoietic function of AA mice,and the mechanism may be related to the Notch signaling pathway.关键词
灸法/艾条灸/贫血,再生障碍性/造血干细胞/Notch信号通路/信号转导/小鼠Key words
Mild Moxibustion/Moxa Stick Moxibustion/Anemia,Aplastic/Hematopoietic Stem Cells/Notch Signaling Pathway/Signal Transduction/Mice分类
医药卫生引用本文复制引用
石健菲,李国娜,赵琛,杨燕萍,戚莉,秦连瑞,焦丹丽,钱雨彤,刘世敏,刘畅,李璟,吴凌翔..温和灸足三里对再生障碍性贫血小鼠造血功能及Notch信号通路相关因子表达的影响[J].针灸推拿医学（英文版）,2026,24(4):330-338,9.基金项目
This work was supported by Project of Clinical Research of Health Industry of Shanghai Municipal Health Commission(上海市卫生健康委员会卫生行业临床研究专项面上项目,No.202340167) （上海市卫生健康委员会卫生行业临床研究专项面上项目,No.202340167）
Shanghai University of Traditional Chinese Medicine Science and Technology Development Project(上海中医药大学科技发展项目,No.24KFL076) （上海中医药大学科技发展项目,No.24KFL076）
High-level Traditional Chinese Medicine Key Subjects of the State Administration of Traditional Chinese Medicine(国家中医药管理局高水平中医药重点学科建设项目,No.zyyzdxk-2023068) （国家中医药管理局高水平中医药重点学科建设项目,No.zyyzdxk-2023068）
Yueyang Hospital Translational Medicine Research Fund Program(岳阳医院"揭榜挂帅"转化医学研究基金项目,No.2024YJJB04). （岳阳医院"揭榜挂帅"转化医学研究基金项目,No.2024YJJB04）
