浙江中医药大学学报2026，Vol.50Issue(7)：811-820,10.DOI:10.16466/j.issn1005-5509.2026.07.001
基于"心应夏"理论探讨TRP热敏通道对慢性心衰大鼠心功能与结构的调控作用
Regulatory Effect of TRP Thermosensitive Channels on Cardiac Function and Structure in Chronic Heart Failure Rats Based on the"Heart Corresponding to Summer"Theory
摘要
Abstract
[Objective]To investigate the effects of temperature changes in summer and winter on cardiac function and structure in rats with chronic heart failure(CHF),and to explore the biological mechanisms underlying the"heart corresponding to summer"phenomenon from the perspective of the thermosensitive transient receptor potential(TRP)-Ca2+-Ras homolog family member A-Rho-associated coiled-coil containing protein kinase(RhoA-ROCK)signaling pathway.[Methods]Taking SD rats as the research subjects,climate-controlled chambers were used to simulate summer and winter temperatures.In each season,the rats were randomly assigned to physiological,CHF,and sham-operated groups.After 8 weeks of feeding,myocardial tissue was retrieved.Cardiac function and structural changes were assessed by echocardiography;myocardial pathological changes were observed after HE staining,the degree of myocardial fibrosis was assessed with Masson's staining;ELISA was used to detect the levels of TRP thermosensitive channel indicators,including myocardial transient receptor potential vanilloid 4(TRPV4),transient receptor potential ankyrin 1(TRPA1),RhoA,ROCK1 and myosin light chain(MLC),and transforming growth factor-β1(TGF-β1);myocardial Ca2+concentration was measured by using the o-cresolphthalein complexone(OCPC)method.[Results]CHF group exhibited seasonal trends in cardiac function and structural alterations,with a seasonal trend where summer was superior to winter.Compared with physiological group and sham-operated group,both TRPV4 and TRPA1 levels in myocardial tissue were upregulated in CHF group;TRPV4 levels were significantly upregulated in summer groups(P<0.01),while TRPA1 levels were significantly upregulated in winter groups(P<0.05).In contrast to cardiac function and structural changes,seasonal rhythms of myocardial Ca2+,RhoA,ROCK1,MLC and TGF-β1 in CHF group exhibited a seasonal trend of lower levels in summer and higher levels in winter.[Conclusion]The biological mechanism of the"heart corresponding to summer"theory may be that seasonal temperature changes activate thermosensitive TRPV4 and TRPA1 channels in myocardial tissue,initiate Ca2+signaling and its downstream RhoA-ROCK signaling pathway,and trigger a series of physiological and pathological effects corresponding to summer.关键词
心应夏/夏冬季节/温度/慢性心力衰竭/TRPV4/TRPA1/RhoA-ROCK信号通路Key words
heart corresponding to summer/summer and winter seasons/temperature/chronic heart failure/TRPV4/TRPA1/RhoA-ROCK signaling pathway分类
医药卫生引用本文复制引用
张文静,黄雪杰,彭宜璐,康帅,申占国,刘晓燕..基于"心应夏"理论探讨TRP热敏通道对慢性心衰大鼠心功能与结构的调控作用[J].浙江中医药大学学报,2026,50(7):811-820,10.基金项目
国家自然科学基金项目(81973716)National Natural Science Foundation Project(81973716) （81973716）
