浙江中医杂志2026，Vol.61Issue(8)：664-668,5.
葛根素调控MMP-2/TIMP-3对大鼠应激性胃溃疡的保护作用研究
Protective Effects of Puerarin Regulating MMP-2/TIMP-3 on Stress-Induced Gastric Ulcer in Rats
摘要
Abstract
Objective:To investigate the protective effects of puerarin on stress-induced gastric ulcer(SGU)in rats by regulating MMP-2/TIMP-3 pathway.Methods:40 SD rats were divided into control group,model group,puerarin low-dose group(50 mg/kg),puerarin high-dose group(100 mg/kg),10 rats in each group.Puerarin low-dose and high-dose groups were given puerarin solution by intraperitoneal injection for 7 days.The control group and model group were given the same volume of normal saline.The stress ulcer model was established by immersion restraint stress method.The gastric motility,gastric mucosal blood flow(GMBF),the degree of gastric mucosal injury and pathological changes of gastric mucosa were observed and recorded.The levels of inflammatory cytokines[interleukin-6(IL-6),interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),serum prostaglandin E2(PGE2)]and oxidative stress factors[superoxide dismutase(SOD),malondialdehyde(MDA),glutathione peroxidase(GSH-Px)]and the expressions of MMP-2 and TIMP-3 in gastric tissue were detected.Results:Compared with the control group,the gastric ulcer area,gas-tric ulcer index(UI),gastric motility indexes,IL-6,TNF-α,IL-1β,PGE2,MDA,MMP-2 levels in the model group were significantly increased(P＜0.05);the levels of GMBF,GSH-Px,SOD and TIMP-3 de-creased significantly(P＜0.05).Compared with the model group,the gastric ulcer area,ulcer index,gastric motility indicators,as well as the levels of IL-6,TNF-α,IL-1β,PGE2,MDA and MMP-2 were signifi-cantly decreased in the puerarin treatment group(P＜0.05);GMBF,GSH-Px,SOD and TIMP-3 were signifi-cantly increased(P＜0.05).Compared with the control group,gastric mucosal erosion,vasodilation,extensive edema and inflammatory cell infiltration were observed in the model group.Compared with the model group,the severity of gastric mucosal lesions,edema and infiltration of inflammatory cells into the submucosa in the Puerarin Treatment group were significantly alleviated.Conclusion:Puerarin has a significant protective ef-fect on SGU in rats by regulating MMP-2/TIMP-3,which can inhibit the inflammatory reaction and oxida-tive stress injury of gastric mucosa in stress-induced rats,improve the microcirculation of gastric mucosa,and reduce the stress-induced gastric mucosal injury.关键词
葛根素/应激性胃溃疡/MMP-2/TIMP-3/胃黏膜损伤/大鼠Key words
puerarin/stress-induced gastric lcer/MMP-2/TIMP-3/gastric mucosal injury/rats引用本文复制引用
刘梦月,刘庆生..葛根素调控MMP-2/TIMP-3对大鼠应激性胃溃疡的保护作用研究[J].浙江中医杂志,2026,61(8):664-668,5.基金项目
浙江省医药卫生科技计划项目(2024KY182) （2024KY182）
