植物遗传资源学报2026，Vol.27Issue(8)：1562-1570,9.DOI:10.13430/j.cnki.jpgr.20260316003
水稻OsWRKY70基因单倍型变异及其与耐冷性的关联分析
Analysis of Haplotype Variation of Rice OsWRKY70 Gene and Its Association with Cold Tolerance
摘要
Abstract
OsWRKY70 is a key transcription factor in rice response to low-temperature stress.However,the genetic mechanism by which its natural variation regulates cold tolerance in rice remains unclear.This study analyzed the haplotype composition,population differentiation,and correlation with cold tolerance of the OsWRKY70 coding region based on"3K rice genomes project".The results showed that 26 SNPs exist in the OsWRKY70 coding region,constituting nine haplotypes,among which Hap1(56.54％)and Hap2(42.24％)were the dominant haplotypes.Population genetic analysis showed significant differentiation of this gene between indica and japonica rice subspecies(Fst=0.598).Hap1 was identified as the characteristic haplotype of indica rice(accounting for 83.10％of indica rice),while Hap2 was the characteristic haplotype of japonica rice(accounting for 98.71％of japonica rice).They contributed 99.98％of the population differentiation index,indicating that this locus is an important site for differentiation between indica and japonica rice.Geographical distribution analysis revealed that Hap1 is mainly distributed in tropical and southern subtropical regions,while Hap2 is more common in high-latitude,high-altitude areas and regions with frequent low temperatures.Cold tolerance phenotypic identification showed that the average survival rate of Hap2(51.86％)was significantly higher than that of Hap1(4.73％),indicating that Hap2 is significantly correlated with cold tolerance in rice seedlings,and this cold tolerance effect is more significant in the genetic context of japonica rice.This study clarified the association between natural variation in OsWRKY70 and differentiation between indica and japonica rice varieties,as well as cold tolerance.Five non-synonymous SNPs found between Hap2 and Hap1 provide a theoretical basis for genetic improvement of cold tolerance and the development of related molecular markers.关键词
水稻/OsWRKY70/耐冷/单倍型Key words
rice/OsWRKY70/cold tolerance/haplotype引用本文复制引用
易崇粉,杨占烈,郭慧,郝留根,王珍珍,张宏伟,甘雨,杨春苗,姜敬伟,向关伦..水稻OsWRKY70基因单倍型变异及其与耐冷性的关联分析[J].植物遗传资源学报,2026,27(8):1562-1570,9.基金项目
贵州省科技计划项目(黔科合基础-ZK[2024]一般557,黔科合支撑[2022]重点028) （黔科合基础-ZK[2024]一般557,黔科合支撑[2022]重点028）
国家水稻产业技术体系(CARS-01) （CARS-01）
国家自然科学基金(32260525) （32260525）
贵州省水稻产业技术体系建设项目(GZSDCYJSTX-202602)Guizhou Provincial Science and Technology Plan(Qiankehe Foundation-ZK[2024]General 557,Qiankehe Support[2022]Key 028) （GZSDCYJSTX-202602）
China Agriculture Research System(CARS-01) （CARS-01）
National Natural Science Foundation of China(32260525) （32260525）
Guizhou Provincial Rice Industry Technology System Construction Projects(GZSDCYJSTX-202602) （GZSDCYJSTX-202602）
