植物遗传资源学报2026，Vol.27Issue(8)：1571-1582,12.DOI:10.13430/j.cnki.jpgr.20260311001
大豆豆芽可溶性糖含量的全基因组关联分析
Genome-wide Association Analysis of Soluble Sugar Content in Soybean Sprouts
摘要
Abstract
To characterize phenotypic variation in soluble sugar content of soybean sprouts and to elucidate its genetic basis,we evaluated 188 improved soybean varieties from the Huang-Huai-Hai region.Soluble sugar content in germinated sprouts was accurately quantified in 2023 and 2024,followed by a genome-wide association study(GWAS)using SNP markers.Substantial phenotypic variation was observed,with soluble sugar content ranging from 1.67 to 20.94 mg/g and a coefficient of variation of 33.33％and 26.73％,indicating considerable genetic diversity for this trait.GWAS detected five loci significantly associated with soluble sugar content[-log10(P)≥5.86]across two years,located on chromosomes 2,10,12,14,and 18.Within these regions,18 candidate genes were identified,with functional annotations enriched in carbohydrate metabolism pathways,including gluconeogenesis and trehalose synthesis,as well as cell wall biosynthesis.Expression analysis further showed that four candidate genes were differentially expressed between high-and low-sugar materials,supporting their potential roles in regulating soluble sugar accumulation.Overall,this study reveals the phenotypic diversity and genetic architecture of soluble sugar content in soybean sprouts from the Huang-Huai-Hai region.The identified loci and candidate genes provide valuable resources for functional marker development and marker-assisted breeding aimed at improving sprout quality.关键词
大豆豆芽/可溶性糖/全基因组关联分析/候选基因Key words
soybean sprouts/soluble sugar/genome-wide association study(GWAS)/candidate genes引用本文复制引用
李艳明,闫龙,吴欣桐,陈强,刘智,史晓蕾,孟子阔,施爱农,李娜,陈雪平..大豆豆芽可溶性糖含量的全基因组关联分析[J].植物遗传资源学报,2026,27(8):1571-1582,12.基金项目
国家大豆产业技术体系(CARS-04-PS09) （CARS-04-PS09）
河北省现代农业产业技术体系(HBCT2024060101) （HBCT2024060101）
河北省农林科学院科技创新专项(2026KJCXZX-LYS-6) （2026KJCXZX-LYS-6）
河北省燕赵黄金台聚才计划骨干人才项目(B2025005024)China Agriculture Research System(CARS-04-PS09) （B2025005024）
Hebei Agriculture Research System(HBCT2024060101) （HBCT2024060101）
HAAFS Agriculture Science and Technology Innovation Project(2026KJCXZX-LYS-6) （2026KJCXZX-LYS-6）
Yan Zhao Golden Platform Talent Gathering Plan of Hebei Province(B2025005024) （B2025005024）
