中药新药与临床药理2026，Vol.37Issue(8)：1436-1442,7.DOI:10.19378/j.issn.1003-9783.2026.08.005
清热逐风合剂对胶原诱导型关节炎大鼠 IL-17A、HIF-1α、VEGF、TGF-β表达的影响
Effects of Qingre Zhufeng Mixture on the Expression of IL-17A,HIF-1α,VEGF,and TGF-β in CIA Rats
摘要
Abstract
Objective To observe the effects of Qingre Zhufeng Mixture on serum interleukin-17A(IL-17A),transforming growth factor-β(TGF-β),and the expression of hypoxia inducible factor-1α(HIF-1α)and vascular endothelial growth factor(VEGF)in synovial tissue of rats with collagen-induced arthritis(CIA),a model of rheumatoid arthritis(RA),and to explore the mechanism by which Qingre Zhufeng Mixture alleviates the inflammatory response in RA.Methods A CIA rat model was established.Methotrexate tablets,tripterygium glycoside tablets,and Qingre Zhufeng Mixture were used as interventions.Paw swelling and arthritis index were measured.Hematoxylineosin(HE)staining was used to observe histopathological changes in synovial tissue.Serum levels of IL-17A and TGF-β were detected by enzymelinked immunosorbent assay(ELISA).The protein expression of HIF-1α and VEGF in synovial tissue was detected by Western Blot.Results Compared with the normal group,the model group showed significantly increased paw swelling and arthritis index(P＜0.01);significantly elevated serum IL-17A level and decreased serum TGF-β level(P＜0.01);and significantly increased protein expression of HIF-1α and VEGF in synovial tissue(P＜0.01).Compared with the model group,the Qingre Zhufeng Mixture group,methotrexate group,and tripterygium glycoside group all showed significantly reduced paw swelling and arthritis index(P＜0.01);significantly decreased serum IL-17A and increased serum TGF-β(P＜0.01);and significantly reduced protein expression of HIF-1α and VEGF in synovial tissue(P＜0.01).Conclusion Regulating the IL-17A/TGF-β balance to promote CD4+T cell differentiation toward regulatory T cells(Tregs),inhibiting the expression of HIF-1α and VEGF,and thereby restoring the Th17/Treg balance may be one of the molecular mechanisms by which Qingre Zhufeng Mixture alleviates the inflammatory response in rheumatoid arthritis.关键词
清热逐风合剂/类风湿关节炎/胶原诱导型关节炎/IL-17A/HIF-1α/VEGF/TGF-β/Th17/Treg/大鼠Key words
Qingre Zhufeng Mixture/rheumatoid arthritis/collagen-induced arthritis/IL-17A/HIF-1α/VEGF/TGF-β/Th17/Treg/rats分类
医药卫生引用本文复制引用
雷海桃,年芳红,王海东,苏小军,田雪梅,吴海娟,杨静,王花,王金海,朱虹,李喜媛,马麦丽娜,马晓飞..清热逐风合剂对胶原诱导型关节炎大鼠 IL-17A、HIF-1α、VEGF、TGF-β表达的影响[J].中药新药与临床药理,2026,37(8):1436-1442,7.基金项目
国家自然科学基金资助项目(82260880) （82260880）
国家中医药管理局高水平中医药重点学科建设基金资助项目(国中医药人教函[2022]226号) （国中医药人教函[2022]226号）
王海东甘肃省名中医传承工作室建设基金资助项目(甘卫中医函[2022]50号) （甘卫中医函[2022]50号）
甘肃省中医药管理局项目(GZKP-2022-13) （GZKP-2022-13）
兰州市青年科技人才创新专项(2023-QN-12) （2023-QN-12）
田雪梅甘肃省名中医传承工作室建设基金资助项目(甘卫中医函[2024]7号) （甘卫中医函[2024]7号）
兰州市科技局资助项目(2024-9-40). （2024-9-40）
