中华中医药学刊2026，Vol.44Issue(8)：6-11,后插3-后插4,8.DOI:10.13193/j.issn.1673-7717.2026.08.002
乳酸菌发酵葛根菌株筛选及协同发酵工艺优化研究
Screening of Lactic Acid Bacteria Strains and Optimization of Synergistic Fermentation Process for Pueraria lobata
摘要
Abstract
Objective This study aimed to screen dominant lactic acid bacteria(LAB)for Gegen(Pueraria lobata)fermenta-tion and optimize the synergistic fermentation processes to enhance the bioactive compound yields.Methods By monitoring the dy-namic changes in bacterial activity,total acid,total flavonoids and antioxidant activity during the fermentation process of six strains of LAB,Analytic Hierarchy Process(AHP)-Entropy Weight Method was used to comprehensively evaluate and screen the domi-nant LAB and optimize the key process parameters such as strain ratio,inoculation amount and solid-liquid ratio based on the synergistic fermentation strategy of dominant bacteria.Results Lactobacillus plantarum P9 and Lactobacillus rhamnosus were the dominant strains for Gegen(Pueraria lobata)fermentation.The optimal process conditions for their synergistic fermentation were a strain ratio of 3∶1,inoculation volume of 10％,solid-liquid ratio of 1∶25,temperature of 37 ℃ and fermentation for 3 d.Un-der these conditions,the viable counts reached 8.96 lgCFU·mL-1(231.6％higher than Lactobacillus plantarum P9 monocul-ture,31.83％higher than Lactobacillus rhamnosus),total acid content peaked at 2.95 g·L-1(91.6％increase over Lactobacil-lus rhamnosus monoculture),and daidzein content achieved 1.32 mg·g-1(164％and 238％enhancements compared to respec-tive monocultures).Conclusion Microbial consortia integration synergistically amplified strain-specific advantages,significantly elevating bioactive metabolites and providing strain selection and process optimization strategy for the development of fermented functional beverages and probiotic preparations based on Gegen(Pueraria lobata).关键词
葛根/乳酸菌/协同发酵/活菌数/总酸/大豆苷元/AHP-熵权法/响应面设计Key words
Gegen(Pueraria lobata)/lactobacillus/complex fermentation/live bacterial count/total acid/daidzein/AHP-Entropy Weight Method/response surface design分类
医药卫生引用本文复制引用
管咏梅,唐烨,李丽琴,董欢欢,潘华平,张华,朱卫丰,臧振中..乳酸菌发酵葛根菌株筛选及协同发酵工艺优化研究[J].中华中医药学刊,2026,44(8):6-11,后插3-后插4,8.基金项目
江西省重点研发计划项目(20224BBG71023) （20224BBG71023）
江西省研究生创新专项(YC2023-S764) （YC2023-S764）
