滨州医学院学报2026，Vol.49Issue(4)：394-399,406,7.DOI:10.19739/j.cnki.issn1001-9510.2026.04.011
海胆碱溶多糖提取方法的优化
Optimization of the extraction method for alkali-soluble polysaccharides from Hemicentrotus pulcherrimus
摘要
Abstract
Objective To systematically optimize the extracting conditions of alkali-soluble polysaccharides from sea urchin gonads.Methods The alkali-soluble polysaccharides were prepared by extraction with 1%NaOH solution,followed by depro-teinization with the Sevag method,dialysis,and freeze-drying.Single-factor experiments were conducted to investigate the effects of key parameters,including extraction temperature,duration,frequency,and solid-to-liquid ratio,according to the yields of al-kali-soluble polysaccharides.An orthogonal experimental design was then employed to comprehensively determine the optimal ex-tracting conditions,which were subsequently validated.Ultimately,a stable and efficient extraction method was established.The total sugar content of crude polysaccharides was determined by the phenol-sulfuric acid method,and the protein content was measured by the BCA method.In addition,the macrophage activation of the alkali-soluble polysaccharides under different extrac-ting methods was evaluated by Griess method.Results The optimal extraction process parameters for alkali-soluble polysaccha-rides were determined as follows:extraction temperature of 20℃,solid-liquid ratio of 1∶30(g/mL),extraction duration of 2 hours,and extraction cycles of 3.Under these conditions,the polysaccharide extraction yield reached 61.19%.The optimized method effectively improved polysaccharide recovery and provided a reliable foundation for subsequent research and development applications.Alkali-soluble polysaccharides obtained under various extraction conditions could significantly activate macrophages at concentrations above 62.5 µg/mL.Conclusion Sea urchin alkali-soluble polysaccharides are free-soluble in water.A high extraction yield(＞60%)can be achieved under mild conditions(temperature≤20℃).Sea urchin alkali-soluble polysaccha-rides have a significant macrophage-activating effect.关键词
海胆多糖/碱溶多糖/正交实验/苯酚-硫酸法/巨噬细胞激活作用Key words
sea urchin polysaccharide/alkali-soluble polysaccharide/orthogonal experiment/phenol-sulfuric acid method/macrophage activation effect分类
医药卫生引用本文复制引用
张甜甜,李阳,张树勋,赵峰..海胆碱溶多糖提取方法的优化[J].滨州医学院学报,2026,49(4):394-399,406,7.基金项目
国家中医药管理局项目(GZY-KJS-SD-2023-095) （GZY-KJS-SD-2023-095）
山东省自然科学基金重点项目(ZR2020KB015 （）
国家自然科学基金(81703391) （81703391）
