草地学报2026，Vol.34Issue(8)：3015-3025,11.DOI:10.11733/j.issn.1007-0435.2026.08.024
不同浓度槲皮素对盐胁迫下罗布红麻种子萌发和幼苗生长的影响
Effects of Different Concentrations of Quercetin on Seed Germination and Seedling Growth of Apocynum venetum L.under Salt Stress
摘要
Abstract
This study investigated the growth regulation mechanism of quercetin on seed germination and seed-ling growth of Apocynum venetum L.under salt stress,and determined its optimal application concentration.This study utilized A.venetum as the experimental material,and established eight treatments:HCKN:200 mmol·L⁻¹ NaCl salt stress;Q1+N-Q6+N:200 mmol·L-1 NaCl+50,100,150,200,250,300 μmol·L-1 quercetin).The study systematically measured seed germination traits(germination rate,germination vigor,germination index),seedling growth indicators(plant height,root growth parameters,biomass,leaf growth parameters),photosynthetic physiological traits(photosynthetic pigment content,chlorophyll fluorescence parameters),and stress resistance physiological indicators(antioxidant enzyme activity,malondialdehyde con-tent,proline content).The alleviation effects were comprehensively evaluated using the membership function method.The results indicated that salt stress had a minor effect on the germination rate of A.venetum seeds but significantly reduced germination vigor and germination index.It severely inhibited the growth of A.vene-tum seedlings and impaired photosynthetic system function,characterized by significantly decreasing photosyn-thetic pigment content and PSⅡ photochemical efficiency,along with increasing accumulation of malondialde-hyde,a lipid peroxidation product.The salt stress-alleviating effect of quercetin exhibited a pronounced concen-tration-dependent response:Low quercetin concentrations(Q1+N,Q2+N)provided limited alleviation,while high concentrations(Q5+N,Q6+N)failed to effectively improve growth and even inhibited A.venetum seed-ling development.Compared to other quercetin concentration treatments,the 150 μmol·L⁻¹ quercetin(Q3+N)treatment showed the best salt stress alleviation effect.Compared to the salt-stressed control(HCKN),it increased seed germination potential and germination index by 2.1％and 3.5％,respectively.Seedling height increased by 97.2％,total chlorophyll content and carotenoid content increased by 17.1％and34.9％respec-tively,proline content increased by 20.6％,and malondialdehyde content decreased by 22.8％(P<0.05).Concurrently,it significantly improved root morphology and chlorophyll fluorescence parameters,bringing seed-ling growth and physiological characteristics closer to the normal control level.Quercetin enhances salt toler-ance in A.venetum seedlings by synergistically promoting photosynthetic pigment synthesis,strengthening anti-oxidant system function,regulating osmotic balance,and optimizing root structure,thereby effectively mitigat-ing salt stress damage.This study identifies the optimal concentration and key physiological mechanisms by which quercetin regulates A.venetum's salt tolerance,providing crucial technical guidance for large-scale culti-vation and ecological restoration of A.venetum in saline-alkali soils.关键词
槲皮素/罗布红麻/盐胁迫/种子萌发/幼苗生长Key words
Quercetin/Apocynum venetum L./Salt stress/Seed germination/Seedling growth分类
农业科技引用本文复制引用
赵雪情,姜黎,赵冰,陈雨芊..不同浓度槲皮素对盐胁迫下罗布红麻种子萌发和幼苗生长的影响[J].草地学报,2026,34(8):3015-3025,11.基金项目
新疆自治区重点研发项目(2023B02045)资助 （2023B02045）
