分析测试学报2026，Vol.45Issue(8)：1785-1796,12.DOI:10.12452/j.fxcsxb.26051401
用于配体靶蛋白鉴定的溶剂诱导蛋白质沉淀方法的发展与应用
Development and Application of Solvent-induced Protein Precipitation Method for Ligand-target Protein Identification
摘要
Abstract
Drugs regulate biological processes by binding to target proteins.Characterizing drug and protein interactions is therefore critical for elucidating mechanisms of action,reducing toxic side ef-fects,overcoming drug resistance,and advancing novel drug discovery.Conventional target identifi-cation methods often require chemical modification of drugs,which tends to disturb native binding in-teractions and produce false-positive results.Solvent-induced protein precipitation(SIP)is a label-free approach that enables high-throughput drug target screening by detecting protein stabilization in-duced upon ligand binding,offering distinct advantages including simple operation and broad appli-cability.In recent years,researchers have developed a series of SIP-derived methods,including SPP,solvent-PISA,iSPP,and the live-cell in situ technique SICFA,which have greatly improved throughput and system compatibility.This review systematically summarizes the fundamental princi-ples,technological evolution,advantages,and limitations of SIP,and highlights its applications in target discovery and validation across cancer,Parkinson's disease,antimicrobial resistance,malar-ia,and other fields.We aim to provide a useful reference for precise target identification and innova-tive drug development.关键词
靶标鉴定/化学免修饰配体/蛋白质组学/液相色谱-质谱联用Key words
target protein identification/chemical modification-free ligand/proteomics/LC-MS/MS分类
化学化工引用本文复制引用
张晓磊,叶玉莹,叶明亮..用于配体靶蛋白鉴定的溶剂诱导蛋白质沉淀方法的发展与应用[J].分析测试学报,2026,45(8):1785-1796,12.基金项目
国家重点研发计划项目(2021YFA1302601) （2021YFA1302601）
辽宁省自然科学基金面上项目(2024-MSBA-64) （2024-MSBA-64）
