辐射研究与辐射工艺学报2026，Vol.44Issue(4)：58-69,12.DOI:10.11889/j.1000-3436.2026-0033
汉防己甲素对放射性肺损伤的保护作用及机制
The protective effect and mechanisms of tetrandrine on radiation-induced lung injury
摘要
Abstract
This study aims to investigate the protective effects of tetrandrine on radiation-induced lung injury(RILI)and elucidate its potential mechanisms.Eighteen mice were randomly assigned to one of three groups:the control group,the irradiation group,and the irradiation plus tetrandrine group.Mice were exposed to a single full thoracic dose of 20 Gy radiation to establish a model of RILI.The lung coefficient was evaluated 12 weeks after radiation.Hematoxylin-eosin staining was used to assess the lung tissue damage.The network pharmacology method was used to screen for potential molecular mechanisms.The protein expression of myeloperoxidase(MPO),F4/80,interleukin 1β(IL1β),interleukin 18(IL18),interleukin 6(IL6),CD86,CD163,NOD-like receptor family pyrin domain containing 3(NLRP3),apoptosis-associated speck-like protein(ASC),and cysteinyl aspartate specific proteinase 1(Caspase1)was assessed using Western blot.The results showed that compared with the control group,the coefficient(1.27±0.05 vs.1.55±0.20),histology score(2.00±1.27 vs.6.00±1.27),and protein expression levels of MPO(1.00±0.21 vs.1.34±0.20),F4/80(1.00±0.13 vs.1.28±0.11),IL1β(1.00±0.18 vs.1.71±0.14),IL6(1.00±0.17 vs.1.62±0.31),and CD86(1.00±0.29 vs.1.62±0.36)increased in the irradiation group,whereas the protein expression of CD163(1.00±0.21 vs.0.59±0.07)was decreased.Compared with the IR group,the coefficient(1.36±0.07),histology score(3.17±1.33),and the protein expression levels of MPO(1.02±0.10),F4/80(0.94±0.23),IL1β(0.97±0.56),IL6(1.01±0.10),and CD86(1.07±0.34)decreased in the IR+Tet group,whereas the protein expression of CD163(0.99±0.25)increased.Network pharmacology analysis identified 93 intersecting genes.KEGG pathway analysis indicated that the intersecting genes were mainly enriched in the NOD-like receptor signaling pathway,PI3K-Akt signaling pathway,and NF-κB signaling pathway.Western blot further confirmed that compared with the control group,the protein expression of NLRP3(1.00±0.24 vs.1.54±0.46)and Caspase1(1.00±0.20 vs.1.66±0.30)was upregulated in the irradiation group;compared with the IR group,the protein expression of NLRP3(1.02±0.25)and Caspase1(0.74±0.37)was downregulated in the IR+Tet group.Tetrandrine can alleviate RILI,possibly by regulating the NLRP3/Caspase1 pathway to suppress M1 macrophage polarization and inhibit inflammatory responses.关键词
汉防己甲素/放射性肺损伤/NLRP3/Caspase1通路/巨噬细胞极化/炎症因子Key words
Tetrandrine/Radiation-induced lung injury/NLRP3/Caspase1 pathway/Macrophage polarization/Inflammatory factor分类
医药卫生引用本文复制引用
乃爱桃,曾慧,李志敏,何贵成,杨巧,廖明初..汉防己甲素对放射性肺损伤的保护作用及机制[J].辐射研究与辐射工艺学报,2026,44(4):58-69,12.基金项目
湖南省自然科学基金项目(2023JJ40588,2023JJ50155,2023JJ40583,2024JJ9374) Supported by the Hunan Provincial Natural Science Foundation of China(2023JJ40588,2023JJ50155,2023JJ40583,and 2024JJ9374) （2023JJ40588,2023JJ50155,2023JJ40583,2024JJ9374）
