辐射研究与辐射工艺学报2026，Vol.44Issue(4)：100-112,13.DOI:10.11889/j.1000-3436.2026-0024
电子加速器辐照对枸杞种子的诱变效应
Mutagenic effects of electron beam irradiation on Lycium barbarum seeds
摘要
Abstract
This study used seeds of Lycium barbarum Ningqi-10,a major cultivated goji variety,as experimental material.Irradiation treatment was conducted using X-rays generated by an electron accelerator at absorbed doses of 0 Gy,80 Gy,100 Gy,150 Gy,200 Gy,and 220 Gy.The effects of different irradiation treatment doses on seed germination and seedling growth characteristics were investigated,and the optimal mutagenic dose was preliminarily determined,thereby establishing a foundation for subsequent screening of M2 generation mutants.During germination,the 80 Gy treatment promoted germination;doses of 100-200 Gy induced a dynamic response pattern characterized by"initial inhibition followed by recovery";and the highest dose(220 Gy)resulted in sustained and significant inhibition.During seedling growth,the inhibitory effect on plant height increased with dose,basal stem diameter was significantly reduced within the 150-220 Gy range,and both leaf nitrogen and chlorophyll contents were significantly decreased across all mutagenic doses.Notably,the 80 Gy treatment led to significant increases in leaf width,leaf area,and leaf roundness,indicating potential for positive morphological variation.Based on a comprehensive evaluation of seed germination,seedling growth vigor,and leaf morphological variation,80 Gy was identified as the optimal mutagenic dose for this variety.Linear regression analysis estimated the median lethal dose(LD 5 0)to be approximately 858 Gy,revealing the exceptionally high radiation tolerance of Ningqi-10.This study established the first dose-response relationship for X-ray irradiation generated by an electron accelerator in this variety,defining its LD 5 0 as approximately 858 Gy and its optimal mutagenic dose as 80 Gy,thereby elucidating its dual characteristics of high tolerance and beneficial low-dose effects,and constructing a variety-specific radiation response pattern.These findings provide critical technical parameters and a scientific basis for mutation breeding and molecular genetic improvement of Lycium barbarum.关键词
电子加速器/枸杞/宁杞10号/种子/生长特性Key words
Electron accelerator/Lycium barbarum/Ningqi-10/Seed/Growth characteristics分类
能源科技引用本文复制引用
闫婷,常书娟,岳静宇,桑昊,师鹏飞,鲁敏,福升,海龙..电子加速器辐照对枸杞种子的诱变效应[J].辐射研究与辐射工艺学报,2026,44(4):100-112,13.基金项目
内蒙古自治区林业科学研究院科研能力提升"揭榜挂帅"项目(2024NLTS02)、2025年度自治区本级科研院所(自然科学类)基本科研业务费项目(2025JKF02) Supported by the Research Capacity Enhancement"Open Competition"Project of Inner Mongolia Academy of Forestry Sciences(2024NLTS02),and the 2025 Annual Basic Research Operating Expenses Project for Natural Science Research Institutes Directly under the Autonomous Region(2025JKF02) （2024NLTS02）
