福建农业科技2026，Vol.57Issue(7)：1-9,9.DOI:10.13651/j.cnki.fjnykj.2026.07.001
菇娘果酚类物质提取工艺优化及其抗氧化和稳定性分析
Optimization of Extraction Process of Phenolic Compounds from Physalis alkekengi and Analysis of Their Antioxidant Activity and Stability
摘要
Abstract
In order to optimize the ultrasonic-assisted extraction process of phenolic compounds from the fruits and persistent calyces of two Physalis cultivars,and to clarify their in vitro antioxidant activity,hypoglycemic related enzyme inhibitory activity and gastrointestinal digestion stability,the mature fruits and calyces of Alkekengi officinarum(red-fruited)and Physalis peruviana(yellow-fruited)were used as the tested materials.The extraction process was optimized by using the single-factor experiments combined with orthogonal array design.The biological activities of phenols in each part were evaluated by DPPH method,ABTS method,α-amylase and α-glucosidase inhibition tests,and the digestion stability was analyzed based on INFOGEST international standardization model.The results showed that the total phenolic concentration of red-fruited calyces was the highest,reaching 62.25 mg GAE·g-1.The optimal extraction conditions were as follows:70%methanol for red fruits,yellow fruits and yellow-fruited calyces,60%methanol for red-fruited calyces,solid-to-liquid ratio of 1∶20(g·mL-1),and ultrasonic treatment for 20-30 min.Among them,the solid-to-liquid ratio was the dominant factor affecting the extraction efficiency.The phenolic extracts of each part exhibited favorable in vitro biological activity,and the red-fruited calyces showed the strongest ABTS+scavenging activity(IC50=0.375 mg·mL-1).The yellow fruit had the strongest DPPH free radical scavenging activity(IC50=0.302 mg·mL-1),and the red fruit had the most significant inhibitory effect on α-glucosidase(IC50=0.472 mg·mL-1)and α-amylase(IC50=0.067 7 mg·mL-1).After in vitro simulated digestion,the retention rate of phenolic activity in the gastric digestion phase was significantly higher than that in the intestinal digestion phase,and each sample still maintained favorable biological activity after complete gastrointestinal digestion.关键词
菇娘果/宿萼/酚类物质/超声辅助提取/抗氧化活性/体外消化Key words
Physalis alkekengi/Persistent calyx/Phenolic compounds/Ultrasonic-assisted extraction/Antioxidant activity/In vitro digestion分类
轻工纺织引用本文复制引用
张艺翔,卢嘉杰,张家瀚,罗岚枫,罗羽洧,单婷婷..菇娘果酚类物质提取工艺优化及其抗氧化和稳定性分析[J].福建农业科技,2026,57(7):1-9,9.基金项目
江苏省大学生创新创业训练计划重点项目(202513573027) （202513573027）
金陵科技学院高层次人才科研启动项目(jit-b-202217). （jit-b-202217）
