福建农业科技2026，Vol.57Issue(7)：33-42,10.DOI:10.13651/j.cnki.fjnykj.2026.07.005
根结线虫侵染下番茄根系产铁载体内生细菌的筛选与鉴定
Screening and Identification of Siderophore-producing Endophytic Bacteria from Tomato Roots Infected by Root-knot Nematodes
摘要
Abstract
Tomato plants,as the highly sensitive crop to iron deficiency stress,are often subjected to the combined infection by root-knot nematodes and soil-borne pathogenic bacteria such as Ralstonia solanacearum.In order to explore the resources of siderophore-producing endophytic bacteria and obtain the biocontrol strains against root-knot nematodes with the potential to antagonize pathogens and mobilize soil nutrients,the Chrome Azurol S(CAS)detection method was used in this study to determine the siderophore-producing ability of 179 strains of endophytic bacteria previously isolated from tomato roots with root-knot nematode disease in the laboratory.The results showed that a total of 20 strains with stable siderophore-secreting activity were obtained from 179 strains of tomato root endophytes,of which 11 strains exhibited siderophore activity at moderate positive level or above.The molecular identification and sequence analysis indicated that the 11 high-yield siderophore-producing strains belonged to genera including Bacillus,Lysinibacillus,Brevibacterium,Xanthomonas and Rhizobium.Among them,the genus Bacillus was the most dominant group,accounting for 63.6%of the high-yield strains.This study enriched the resource reserve of endophytic siderophore-producing bacteria from plant sources,and provided a reference for the further research on the interaction mechanisms among plant beneficial bacteria,Ralstonia solanacearum and root-knot nematodes,as well as the development of novel nematode biocontrol agents with pathogen-antagonistic functions.关键词
根结线虫/番茄/内生菌/铁载体/筛选/分子鉴定Key words
Root-knot nematode/Tomato/Endophytic bacteria/Siderophore/Screening/Molecular identification分类
农业科技引用本文复制引用
梁锐鑫,吴寒,王林娟,杨秋颖,张昱辰,李欣,田宝玉..根结线虫侵染下番茄根系产铁载体内生细菌的筛选与鉴定[J].福建农业科技,2026,57(7):33-42,10.基金项目
福建省自然科学基金项目(2026J001371) （2026J001371）
国家自然科学基金项目(31670125). （31670125）
