福建农业科技2026，Vol.57Issue(7)：57-65,9.DOI:10.13651/j.cnki.fjnykj.2026.07.008
番茄根内生菌中产木质纤维素酶菌株筛选
Screening of Lignocellulase-producing Strains from Endophytic Bacteria of Tomato Roots
摘要
Abstract
The Plant endophytes with abundant diversity are the excellent strain resources for developing new microbial fertilizers and green biocontrol agents.In order to explore the endophytic functional strains of tomato roots with strong root colonization potential,and to clarify the correlation mechanism between the lignocellulose degradation ability of endophytic bacteria and root colonization,135 strains of endophytic bacteria in tomato roots preserved in the laboratory were used as the materials in this study.The cellulase and xylanase activities of the strains were qualitatively detected by using the transparent circle method with CMC and xylan plates through primary and secondary screening,so as to systematically evaluate their lignocellulose degradation potential.The results showed that most of the tested strains had lignocellulose degradation activity.Among them,67.4%of the strains had cellulase activity,68.8%of the strains had xylanase activity,and the activity of the two enzymes was highly synergistic,which met the needs of compound degradation of plant cell wall.The identified strains of Bacillus and Pseudomonas generally exhibited the enzyme-producing ability.These two types of bacteria were the main sources of microbial inoculants at present.It was confirmed that their excellent degradation ability was closely related to the colonization performance of rhizosphere and root.In summary,lignocellulose-degrading ability was widely present in tomato endophytes,which was an important basis for microorganisms to adapt to the root microenvironment and achieve the persistent colonization.This property could be adopted as a vital evaluation index for rapid screening of high-efficiency endophytic biocontrol strains.关键词
纤维素酶/木聚糖酶/植物根内生菌/定殖/微生物菌剂Key words
Cellulase/Xylanase/Plant root endophytes/Colonization/Microbial inoculants分类
农业科技引用本文复制引用
王林娟,张艳红,梁锐鑫,杨秋颖,张昱辰,李欣,田宝玉..番茄根内生菌中产木质纤维素酶菌株筛选[J].福建农业科技,2026,57(7):57-65,9.基金项目
福建省自然科学基金面上项目(2026J001371) （2026J001371）
国家自然科学基金项目(31670125). （31670125）
