福建农业科技2026，Vol.57Issue(7)：66-73,8.DOI:10.13651/j.cnki.fjnykj.2026.07.009
大肠杆菌cysK基因敲除和过表达对庆大霉素的耐受性分析
Tolerance Analysis of cysK Gene Knockout and Overexpression in Escherichia coli to Gentamicin
摘要
Abstract
The widespread use of antibiotics has led to a global problem of bacterial resistance,and weakened the therapeutic effect of antibiotics.In order to search for the new targets for antibiotic action,the bactericidal effects of aminoglycoside antibiotics,β-lactam antibiotics,and quinolone antibiotics on the cysK gene knockout strain(ΔcysK)of Escherichia coliwere detected,and the bactericidal effect of gentamicin on the cysK gene overexpressing strain ΔcysK-pCA24N(cysK)of Escherichia coli was further tested.Finally,the bactericidal effect of gentamicin on the cysK gene knockout strain(ΔcysK)of Escherichia coli was tested by the exogenous addition of a low concentration of cysteine.The results showed that compared with the wild-type Escherichia coli(WT),the cysK gene knockout strain(ΔcysK)of Escherichia coli had a tolerance phenotype to aminoglycoside antibiotics,β-lactam antibiotics,and quinolone antibiotics.In addition,the cysK gene overexpressing strain ΔcysK-pCA24N(cysK)of Escherichia coli restored its sensitivity to gentamicin,indicating that the cysK gene played a role in the resistance of bacteria to aminoglycoside antibiotics.The exogenous addition of a low concentration of cysteine could restore the sensitivity of the cysK gene knockout strain(ΔcysK)of Escherichia coli to gentamicin,but the exogenous addition of high concentration of cysteine would aggravate the tolerance phenotype of the cysK gene knockout strain(ΔcysK)of Escherichia coli to gentamicin.Therefore,the reason why the knockout of cysK gene led to the tolerance of bacteria to gentamicin may not be simply the lack of cysteine,and the further in-depth research was worthy of attention.The above results confirmed that the cysK gene had a significant effect on the tolerance of Escherichia coli to gentamicin,and could be used as a new target for antibiotic action.关键词
大肠杆菌cysK基因/细菌耐药/庆大霉素/半胱氨酸途径Key words
cysK gene of Escherichia coli/Bacterial resistance/Gentamicin/Cysteine pathway分类
生物科学引用本文复制引用
陈稼骏,王声婵,陈燕华,王妍..大肠杆菌cysK基因敲除和过表达对庆大霉素的耐受性分析[J].福建农业科技,2026,57(7):66-73,8.基金项目
福建省自然科学基金面上项目(2026J001374). （2026J001374）
