分析测试学报2026，Vol.45Issue(8)：1706-1712,7.DOI:10.12452/j.fxcsxb.26030401
基于透射电镜与扫描电镜的多源外泌体形貌表征分析
Morphological Characterization and Analysis of Multi-source Exosomes Based on Transmission Electron Microscopy and Scanning Electron Microscopy
摘要
Abstract
As an important category of extracellular vesicles,exosomes have extended their research scope from mammalian cells to the fields of plants and microorganisms,emerging as a novel focus for cross-species information communication and biomedical research and applications.However,the fundamental physical properties of exosomes from different biological sources,especially the hetero-geneity in morphology and size,have not yet been systematically compared.In this study,transmis-sion electron microscopy(TEM)and scanning electron microscopy(SEM)were employed to conduct multi-dimensional morphological characterization of nine types of exosomes derived from three distinct biological sources:cell sources(human bladder cancer cell lines EJ and 5637,human umbilical cord mesenchymal stem cells hUC-MSC);plant sources(Camptotheca acuminata,Artemisia annua,Polygonatum odoratum);microbial sources(Escherichia coli ATCC 25922,DH5α,Clostridium dif-ficile).TEM imaging and particle size statistical analysis revealed significant differences in morpholo-gy,average particle size,and distribution uniformity among the particles from different sources.SEM results further verified their three-dimensional morphological characteristics.This study estab-lishes a morphological benchmark for the characterization of cross-species exosomes,and provides key physical parameters for understanding the similarities and differences in their biogenesis mecha-nisms and supporting their subsequent functional applications.关键词
外泌体/透射电子显微镜/形貌表征/粒径分析/多源对比Key words
exosomes/transmission electron microscopy/morphological characterization/parti-cle size analysis/multi-source comparison分类
化学化工引用本文复制引用
姜莹,张兆伟,刘定斌..基于透射电镜与扫描电镜的多源外泌体形貌表征分析[J].分析测试学报,2026,45(8):1706-1712,7.基金项目
国家自然科学基金资助项目(22425401,82373115) （22425401,82373115）
南开大学2025年科研仪器实验技术研发项目(25NKSYJS02) （25NKSYJS02）
