神经损伤与功能重建2026，Vol.21Issue(8)：450-456,7.DOI:10.16780/j.cnki.sjssgncj.20260191
失眠障碍患者外周淋巴细胞亚群特征与其认知功能的相关性研究
Association of Peripheral Lymphocyte Subset Profiles with Cognitive Function in Patients with Insomnia Disorder
摘要
Abstract
Objective:To investigate the characteristics of peripheral lymphocyte subsets in patients with insomnia disorder(ID)and their associations with cognitive function.Methods:A total of 39 drug-naïve patients with ID and 51 healthy controls(HCs)were recruited from Wuhan First Hospital between April 2022 and March 2023.Cognitive function was assessed using the Montreal Cognitive Assessment(MoCA)and the Stroop Color-Word Test.Flow cytometry was performed to determine the proportions and absolute counts of peripheral lymphocyte subsets in both groups.Between-group comparisons,correlation analyses,and multivariable linear regression analyses were conducted to evaluate the relationships between peripheral lymphocyte subsets and cognitive function.Results:Compared with HCs,the ID group had significantly lower MoCA total scores and poorer performance in multiple cognitive domains(P＜0.05).In the Stroop test,the ID group showed reduced accuracy and prolonged Stroop-D reaction time(P＜0.05).In addition,the ID group exhibited significantly increased proportions and absolute counts of T cells,helper T lymphocytes(Th cells),B cells,and total T+B+NK cells(P＜0.05),whereas the proportion of NK cells was significantly decreased(P=0.001).Correlation analyses further showed that the MoCA total score and several domain scores were negatively correlated with the proportions and absolute counts of Th cells and B cells(P＜0.05),but positively correlated with the proportion of NK cells(P＜0.05).Moreover,the accuracy and reaction time in Stroop-C and Stroop-D were significantly correlated with T-cell-and Th-cell-related parameters(P＜0.05).Multivariable linear regression analysis showed that,after adjustment for age,education level,and BMI,Th-cell proportion,Th-cell absolute count,the Th/cytotoxic T lymphocyte(CTL)ratio,B-cell absolute count,NK-cell proportion,and total T+B+NK absolute count were independently associated with the MoCA total score.Conclusion:Patients with ID showed mild multidomain cognitive impairment,predominantly involving executive function,accompanied by a peripheral immune profile characterized by adaptive immune predominance.This imbalance in peripheral lymphocyte subsets was significantly associated with cognitive decline and may represent a potential biomarker of insomnia-related cognitive impairment.关键词
失眠障碍/认知障碍/淋巴细胞亚群Key words
insomnia disorder/cognitive function/lymphocyte subsets分类
医药卫生引用本文复制引用
宋昕桦,高子唅,王广,娄敏杰,张一可,刘欣,梅俊华,陈国华..失眠障碍患者外周淋巴细胞亚群特征与其认知功能的相关性研究[J].神经损伤与功能重建,2026,21(8):450-456,7.基金项目
湖北省中医药管理局中医药科研项目(黄连解毒汤调节Homer1a改善突触稳态预防慢性睡眠剥夺加重阿尔茨海默病进展的机制研究,No.ZY2025L039) （黄连解毒汤调节Homer1a改善突触稳态预防慢性睡眠剥夺加重阿尔茨海默病进展的机制研究,No.ZY2025L039）
武汉市自然科学基金探索计划(晨光计划)市属医疗机构临床研究重点专项(基于睡眠微结构及功能磁共振探讨舒肝调心方治疗卒中后抑郁合并失眠的临床疗效及机制研究,No.2025020701020233) （晨光计划）
