高等学校化学学报2026，Vol.47Issue(9)：123-131,9.DOI:10.7503/cjcu20260103
一维DNA纳米材料自组装效率的可视化测量
Visualized Measurement of Self-assembly Efficiency in One-dimensional DNA Nanomaterials
摘要
Abstract
One-dimensional(1D)DNA nanomaterials exhibit broad potential for applications across multiple fields.However,quantitative evaluation of their self-assembly efficiency remains challenging.In this work,six-helix bundle(6HB)DNA fibers were employed as a model system.Building upon existing image analysis methods,we developed an integrated and parameterized approach to overcome the challenges of single-particle identification and counting in high-aspect-ratio,entangled 1D fibers,and established an AFM image-based method for visualizing and quantifying self-assembly efficiency.In this method,the area coverage ratio and contour length density extracted from AFM images were used as quantitative parameters to characterize 1D DNA fiber networks.Using this method,the effects of buffer pH(5-11)and Mg²⁺ concentration(6.25-87.5 mmol/L)on the assembly behavior of 6HB DNA fibers were investi-gated.The results show that efficient assembly of 6HB DNA fibers occurs within a well-defined window of conditions.Continuous long fibers are preferentially formed in the pH range of 6-8,with the highest assembly efficiency at pH=8,where the area coverage ratio reaches ca.36%,and the contour length density is ca.8 μm/μm².When the pH value deviates from this range,the continuous 1D network gradually transforms into fragmented or disordered aggregates,and both parameters decrease to background levels.Fiber assembly also shows a clear threshold response to Mg²⁺ concentration:the assembly efficiency increases rapidly above 25 mmol/L,and stable long fibers form at con-centrations above 50 mmol/L,corresponding to area coverage ratios>20%and contour length densities>4 μm/μm².These results quantitatively demonstrate the strong dependence of DNA nanostructure self-assembly on environmental conditions such as pH and ionic strength.The analytical framework established here provides a useful quantitative tool for the design,assembly,and application of 1D DNA nanomaterials.关键词
六螺旋束DNA纤维/原子力显微镜图像分析/自组装效率Key words
Six-helix bundle DNA fibers/AFM image analysis/Self-assembly efficiency分类
化学化工引用本文复制引用
刘雨庆,程杰,王越,焦凯,诸颖,李江,郭琳洁..一维DNA纳米材料自组装效率的可视化测量[J].高等学校化学学报,2026,47(9):123-131,9.基金项目
国家重点研发计划项目(批准号:2023YFC3404200)和国家自然科学基金(批准号:22325406,22525605,32301185)资助.Supported by the National Key Research and Development Program of China(No.2023YFB3208200)and the National Natural Science Foundation of China(Nos.22325406,22525605,32301185) （批准号:2023YFC3404200）
