寒旱农业科学2026，Vol.5Issue(8)：765-771,7.DOI:10.3969/j.issn.2097-2172.2026.08.013
单瓣矮牵牛试管花芽诱导及试管成花影响因素的研究
Study on the in vitro Floral Bud Induction and Factors Affecting the in vitro Flowering in Single-petaled Petunia
摘要
Abstract
To develop new in vitro flowering products that are easy to prepare,rich in flower color,and low in malformation rate,the single-petaled petunia variety Dream(pink-veined),was first used as a model to investigate the effects of photoperiod,light intensity,temperature,sucrose concentration in the medium,and in-bottle humidity on flower bud induction and flowering.Subse-quently,the optimized and integrated protocol were applied to 6 differently colored varieties,including Dream(pink-veined),Melody(purple-red),Super Cascade(red),Pan American Mirage(blue),Million Bells(also known as Calibrachoa,orange),and Million Bells(also known as Calibrachoa,yellow)to validate its universality.Results showed that single-petaled petunia plants could successfully induced flower buds and bloomed normally in Erlenmeyer flasks when cultured in MS medium containing 40 g/L sucrose at pH 6.0,sealed with Zhentai gas-permeable membranes,under a photoperiod of 16 h light and 8 h dark,a light intensity of 2 000 lx,and a tem-perature of 25℃.The plants exhibited continuous flowering within the flask for up to 30 d.All 6 differently colored varieties success-fully completed the flowering process,forming miniature landscapes with multiple flower colors.This study established a stable and universal standardized protocol for producing in vitro flowering plants.The protocol is hormone-free,operationally reliable,and also provides an ideal model for studying plant reproductive development and flower color regulation.关键词
单瓣矮牵牛/试管花芽诱导/组织培养/试管成花/微型景观Key words
Single-petaled petunia/in vitro floral bud induction/Tissue culture/in vitro flowering/Miniature landscape分类
农业科技引用本文复制引用
厚毅清,裴怀弟,李静雯,王立光,陈玉梁..单瓣矮牵牛试管花芽诱导及试管成花影响因素的研究[J].寒旱农业科学,2026,5(8):765-771,7.基金项目
国家现代农业产业技术体系(CARS-21) （CARS-21）
甘肃省农业科学院科技成果转化项目(2025GAAS-CGZH06、2026GAAS-CGZH07). （2025GAAS-CGZH06、2026GAAS-CGZH07）
