寒旱农业科学2026，Vol.5Issue(8)：780-784,5.DOI:10.3969/j.issn.2097-2172.2026.08.015
禽蛋中金刚烷胺和4种氟喹诺酮类药物残留检测方法的建立
Establishment of the Method for Detecting Amantadine and 4 Fluoroquinolone Residues in Poultry Eggs
摘要
Abstract
A liquid chromatography-mass spectrometry method was established for the simultaneous determination of amanta-dine and 4 fluoroquinolone drugs,namely enrofloxacin,ciprofloxacin,danofloxacin,and sarafloxacin,in poultry eggs,providing a refer-ence for the rapid and convenient determination of veterinary drug residues in poultry eggs.Hen egg samples were extracted with Na2EDTA solution and formic acid-acetonitrile aqueous solution,followed by vortexing and high-speed centrifugation.The superna-tant was purified using a Agilent Captiva EMR-Lipid extraction cartridge,concentrated under nitrogen,and then analyzed using an aqueous solution containing 1 g/kg formic acid and a 1 g/kg formic acid-acetonitrile aqueous solution as mobile phases to determine residues of amantadine and the 4 fluoroquinolone drugs in eggs.Results showed that within the linear range of 1 to 50 μg/kg,the lin-ear correlation coefficients of amantadine and the fluoroquinolone veterinary drugs were all above 0.99.The relative standard deviation of amantadine ranged from 0.20%to 0.58%,while that of the fluoroquinolones ranged from 0.06%to 0.55%.The average spiked recov-eries of the 5 drugs ranged from 89.3%to 111.0%.The chromatograms further confirmed that the adopted chromatographic conditions could accurately detect amantadine and the 4 fluoroquinolone drugs in eggs.This method is simple,rapid,accurate,and highly repro-ducible,making it suitable for the determination of poultry egg samples and applicable as a routine laboratory method for poultry egg testing.关键词
禽蛋/氟喹诺酮类/金刚烷胺/液相色谱-质谱联用法Key words
Poultry egg/Fluoroquinolone/Amantadine/Liquid chromatography-mass spectrometry分类
农业科技引用本文复制引用
孔庆德..禽蛋中金刚烷胺和4种氟喹诺酮类药物残留检测方法的建立[J].寒旱农业科学,2026,5(8):780-784,5.基金项目
武威市市级科技计划B类项目(WW25Z01NY033). （WW25Z01NY033）
