环境工程学报2026，Vol.20Issue(8)：2499-2510,12.DOI:10.12030/j.cjee.202512079
四环素胁迫下群体感应对苦草附生生物膜中抗生素抗性基因的调控作用
Regulation of quorum sensing on the antibiotic resistance genes in epiphytic biofilm of Vallisneria spiralis under tetracycline stress
摘要
Abstract
To elucidate the regulatory mechanisms of quorum sensing(QS)on antibiotic resistance genes(ARGs)dissemination in the epiphytic biofilms of Vallisneria spiralis under tetracycline(TC)stress,a bidirectional modulation system was established by exogenous addition of N-acyl homoserine lactones(AHLs)and quorum sensing inhibitors(QSIs).This approach was combined with metagenomic sequencing and extracellular polymeric substances(EPS)analysis.The results demonstrated that different QS regulators exerted diametrically opposite effects:AHLs(C6-HSL/C8-HSL)significantly promoted EPS secretion,whereas QSIs(vanillin/furanone)inhibited EPS secretion(P<0.05).Metagenomic analysis identified over 400 ARG subtypes belonging to 15 classes,with multi-drug resistance(118 types)and β-lactam(101 types)resistance genes being the most diverse.Host analysis revealed that AHLs significantly reduced the relative abundance of ARGs(e.g.,β-lactams and quinolones)by decreasing the abundance of their bacterial hosts(P<0.05).Conversely,QSIs significantly increased ARG relative abundance through host enrichment.Furthermore,correlation analysis demonstrated that the proportion of positive correlations between QS core genes and ARGs was notably higher in the QSIs groups(58.97%)compared to the AHLs groups(50.00%).This study elucidated the dual mechanisms underlying QS-mediated regulation of ARGs dissemination:on one hand,QS signaling molecules drive ecological niche competition within the microbial community,reducing the relative abundance of potential ARGs hosts;on the other hand,the QS system strengthens the physical defense barrier of biofilms by upregulating EPS secretion,thereby effectively reducing the risk of ARG dissemination.关键词
群体感应/抗生素抗性基因/附生生物膜/胞外聚合物/调控机制Key words
quorum sensing/antibiotic resistance genes/epiphytic biofilm/extracellular polymeric substances/regulatory mechanism分类
资源环境引用本文复制引用
韩宇,张焕军,王庆德,李轶..四环素胁迫下群体感应对苦草附生生物膜中抗生素抗性基因的调控作用[J].环境工程学报,2026,20(8):2499-2510,12.基金项目
国家重点研发计划资助项目(2024YFC3212600) （2024YFC3212600）
国家自然科学基金资助项目(U25A20370) （U25A20370）
