医学分子生物学杂志2026，Vol.23Issue(4)：382-388,7.DOI:10.3870/j.issn.1672-8009.2026.04.002
miR-370-3p靶向STAT3对肝癌细胞HepG2迁移、侵袭能力的影响
Effect of miR-370-3p on Migration and Invasion of Hepatocellular Carcinoma Cells HepG2 via Targeting STAT3
摘要
Abstract
Objective To explore the influence of miR-370-3p on migration and invasion of hepatocellular carcinoma HepG2 cells by targeting STAT3.Methods The miR-NC or miR-370-3p mimic and pcDNA or pcDNA-STAT3 were transfected into HepG2 cells,and the mRNA relative ex-pression levels of miR-370-3p and STAT3 were detected.miR-370-3p mimic and STAT3-wild type(wt)or STAT3-mutant(mut)were co-transfected into HepG2 cells.Finally,the control group,miR-370-3p group,STAT3 group and miR-370-3p+STAT3 group were established.Transwell assay and Wound-healing assay were applied to detect the invasion,migration of cells.Western blot assay was applied to detect the expression level of epithelial-mesenchymal transition associated pro-teins.The orthotopic xenograft mouse model was established,and mice were divided into miR-NC group and miR-370-3p group.Results Compared with the control group,the relative expression level of miR-370-3p was significantly upregulated in the miR-370-3p mimic group.In the pcDNA-STAT3 group,the relative expression level of STAT3 mRNA was significantly upregulated.Overexpression of miR-370-3p inhibits invasion,migration,and affect the expression of epithelial-mesenchymal transition associated pproteins in HepG2 liver cancer cells.Additionally,miR-370-3p targets STAT3 to suppress the aforemen-tioned metastatic capabilities of HepG2 liver cancer cells.In in vivo experiments,overexpression of miR-370-3p inhibits tumor growth.Conclusion miR-370-3p can inhibit the invasion,migration of hepatocel-lular carcinoma cells HepG2 by targeting STAT3.关键词
miR-370-3p/STAT3/肝癌/侵袭/迁移/上皮间质转化Key words
miR-370-3p/STAT3/hepatocellular carcinoma/invasion/migration/epitheli-al-mesenchymal transition分类
医药卫生引用本文复制引用
刘雅婧,贾仕婵,姚书鹏..miR-370-3p靶向STAT3对肝癌细胞HepG2迁移、侵袭能力的影响[J].医学分子生物学杂志,2026,23(4):382-388,7.基金项目
2024年度院级教育教学改革研究项目(AZJKY2024035) This work was supported by a grant from the 2024 Annual College-level Educational Reform Research Project(AZJKY2024035). （AZJKY2024035）
