医学分子生物学杂志2026，Vol.23Issue(4)：406-411,6.DOI:10.3870/j.issn.1672-8009.2026.04.005
miR-429通过Wnt/β-catenin通路影响慢性难愈合创面修复
miR-429 Affects Chronic Non-Healing Wound Repair through Wnt/β-catenin Pathway
摘要
Abstract
Objective To investigate the effects of miR-429 on the healing of chronic non-heal-ing wounds and the Wnt/β-catenin signaling pathway.Methods A total of 60 rats were randomly divided into 5 groups:blank control group,model group,negative control group(injected with antagomir NC),LV-429 group(injected with miR-429 antagomir lentivirus),and inhibitor group(injected with miR-429 antagomir lentivirus and Wnt/β-catenin signaling pathway inhibitor IWP-2).The wound healing rates in rats were assessed,and wound tissue samples were collected for measurement of miR-429 levels,as well as the protein expression of Wnt3a and β-catenin,and the expression of vascular endothelial growth factor A(VEGFA)and endothelial nitric oxide synthase(ENOS).Rat serum samples were collectedor measurement of the levels of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α).Results Compared with that in the model group and the negative control group,the miR-429 expression level was reduced in the LV-429 group and the in-hibitor group.Compared with the model group and the negative control group,in the LV-429 group,the wound healing rates,Wnt3a,β-catenin protein expression,and the positive expression rates of VEGFA and ENOS were all increased,while the IL-6 and TNF-α levels were both decreased(P＜0.05).In the inhibitor group,the wound healing rate,Wnt3a and β-catenin protein expression,and the positive expression rates of VEGFA and ENOS were all decreased,while the IL-6 and TNF-α levels were both increased(P＜0.05).Conclusion Inhibiting the expression of miR-429 can promote the repair of chronic refractory wounds,and this process is mediated by activating the Wnt/β-catenin signaling pathway.关键词
miR-429/创面修复/Wnt/β-catenin通路Key words
miR-429/wound repair/Wnt/β-catenin signaling pathway/vascular endothelial growth factor A/endothelial nitric oxide synthase/wound healing rate/interleu-kin-6/tumor necrosis factor-α分类
医药卫生引用本文复制引用
黄文文,孟丽,谢辉,胡寿勇,石华峰..miR-429通过Wnt/β-catenin通路影响慢性难愈合创面修复[J].医学分子生物学杂志,2026,23(4):406-411,6.基金项目
荆门市科学技术局项目(No.2022YFZD218) This work was supported by a grant from the Jingmen Municipal Bureau of Science and Technology(No.2022YFZD218). （No.2022YFZD218）
