医学分子生物学杂志2026，Vol.23Issue(4)：445-451,7.DOI:10.3870/j.issn.1672-8009.2026.04.011
利福平调控miR-28-5p/Nrf2信号轴对肝细胞损伤的影响
Effect of Rifampicin on Hepatocyte Injury by Regulation of miR-28-5p and Nrf2
摘要
Abstract
Objective To explore the effect of rifampicin on miR-28-5p/nuclear factor-ery-throid 2-related factor 2(Nrf2)signaling and liver cell injury.Methods L02 cells were divided into 6 groups:control group,low-,medium-,and high-dose rifampicin groups,miR NC group,and miR-28-5p inhibitor group.The expression level of miR-28-5p,cell viability,apoptosis rate,inflammatory factor levels,superoxide dismutase(SOD),malondialdehyde(MDA),aspartate aminotransferase(AST),and alanine aminotransferase(ALT)in each group of L02 cells were detected.Meanwhile,the targeting relationship between miR-28-5p and Nrf2 in L02 cells was ana-lyzed.Results The expression level of miR-28-5p in L02 cells were increased with the increase of rifampicin concentration.Compared with those in the control group,the viability of L02 cells in the low-,medium-,and high-dose rifampicin groups,miR NC group,and miR-28-5p inhibitor group was decreased,the apoptosis rate was increased,the levels of inflammatory factors,MDA,AST,and ALT were increased,and the levels of SOD and Nrf2 were decreased.Compared with those in the miR NC group,the viability of L02 cells in the miR-28-5p inhibitor group was increased,the apoptosis rate was decreased,the levels of inflammatory factors,MDA,AST,and ALT were de-creased,and the levels of SOD and Nrf2 were increased.miR-28-5p could targetly inhibit the ex-pression of Nrf2.Conclusion Rifampicin can promote liver cell injury by miR-28-5p and inhibition of Nrf2.关键词
核转录因子红系2相关因子2/利福平/miR-28-5p/凋亡/肝细胞损伤Key words
nuclear factor-erythroid 2-related factor 2/rifampicin/miR-28-5p/apoptosis/hepatocyte injury分类
医药卫生引用本文复制引用
陈振华,王林,梅湘,邹芳芳..利福平调控miR-28-5p/Nrf2信号轴对肝细胞损伤的影响[J].医学分子生物学杂志,2026,23(4):445-451,7.基金项目
湖南省自然医卫行业联合基金(No.2024JJ9475) This work was supported by a grant from the Hunan Province Natural Medicine and Healthcare Industry Joint Fund(No.2024JJ9475). （No.2024JJ9475）
