广西科学院学报2026，Vol.42Issue(2)：119-132,14.DOI:10.13657/j.cnki.gxkxyxb.20260819.001
微生物几丁质脱乙酰酶的研究进展
Research Progress of Microbial Chitin Deacetylase
摘要
Abstract
Chitin is the second most abundant natural polymeric polysaccharide in nature,ranking only behind cellulose.Its deacetylated product,chitosan,is highly favored in industrial production due to its excellent physicochemical properties.Chitin deacetylase(CDA)is capable of converting chitin into chitosan with en-hanced bioactivity under mild reaction conditions.The enzymatic process features green sustainability,high efficiency,excellent substrate specificity,and low energy requirements,and has therefore become a hot spot of current research in the field of chitin deacetylation.However,CDA has a series of problems such as low en-zyme activity,susceptibility to product feedback inhibition,and low catalytic efficiency for crystalline chitin,which seriously restricts its large-scale industrial application.Therefore,this article reviews the research pro-gress of CDA since the 1980s,focusing on the development process,enzymatic properties and deacetylation mechanism of CDA,and discusses the application of chitosan,the product of chitin catalyzed by CDA.Finally,potential approaches for improving the catalytic performance and expression efficiency of CDA were prospec-ted from the aspects of mining novel enzymes from extreme environments,computer-aided design and trans-formation of the enzyme active center,alleviation of product inhibition,and regulation of fermentation proces-ses,so as to provide a theoretical reference for the efficient bioconversion and utilization of chitin resources.关键词
几丁质/壳聚糖/几丁质脱乙酰酶/脱乙酰模式/催化机制Key words
chitin/chitosan/chitin deacetylase/pattern of deacetylation/catalytic mechanism分类
化学化工引用本文复制引用
张红岩,谢俊杰,闫祎哲,韦玉玲,申乃坤..微生物几丁质脱乙酰酶的研究进展[J].广西科学院学报,2026,42(2):119-132,14.基金项目
国家自然科学基金项目(32560023),广西"尖峰"行动计划项目(桂科JF2504850012)和广西民族大学大学生创新创业训练计划项目(202410608026,202310608017,S202510608309)资助. （32560023）
