广西科学院学报2026，Vol.42Issue(2)：143-155,13.DOI:10.13657/j.cnki.gxkxyxb.20260819.003
拮抗金黄色葡萄球菌植物乳植杆菌的筛选鉴定及发酵条件优化
Screening of Lactic Acid Bacteria with Antagonistic Activity Against Staphylococcus aureus and Optimization of Fermenta-tion Conditions
摘要
Abstract
In order to screen lactic acid bacteria exhibiting potent antagonistic effects against Staphylococcus aureus and to optimize their fermentation parameters,S.aureus was employed as the indicator organism.Strain identification was performed based on morphological characteristics,physiological and biochemical properties,and 16S rRNA gene sequencing,followed by assessment of their antibacterial activity.The fermen-tation conditions for antimicrobial compound production were subsequently optimized via single-factor tests and orthogonal experimental design.As a result,a lactic acid bacterial strain S13 with potent antagonistic ac-tivity against S.aureus was obtained and identified as Lactiplantibacillus plantarum.The Cell-Free Superna-tant(CFS)of this strain exhibited antibacterial activity against S.aureus within a pH value range of 3.0-7.0 and demonstrated thermostability at temperatures ranging from 40℃to 121℃.Moreover,the antibacte-rial substances present in the CFS could be completely eliminated by various proteases,preliminarily indica-ting that the antimicrobial compounds produced by this strain are proteinaceous bacteriocins.Through opti-mization,the optimal fermentation conditions for the production of antibacterial substances by strain S13 were determined as follows:initial pH value 6.5,incubation temperature 36℃,fermentation duration 24 h,inoculum concentration 3％(V/V),sucrose as the carbon source at 20 g/L,corn steep liquor as the nitrogen source at 19 g/L,manganese sulfate as the inorganic salt at 9.25 g/L,and Tween 80 at a concentration of 3 mL/L.Under the optimized fermentation conditions,the inhibitory activity of strain S13 CFS was enhanced by 14.61％relative to that prior to optimization.The results indicate that the screened L.plantarum S13 ex-hibits good antagonistic activity against S.aureus,possesses high safety,and can serve as a novel food pre-servative strain resource.关键词
金黄色葡萄球菌/植物乳植杆菌/拮抗菌/正交试验/发酵条件优化Key words
Staphylococcus aureus/Lactiplantibacillus plantarum/antagonistic bacteria/orthogonal test/op-timization of fermentation conditions分类
轻工纺织引用本文复制引用
蒙展彰,黄锦华,原宝龙,张红岩,申乃坤..拮抗金黄色葡萄球菌植物乳植杆菌的筛选鉴定及发酵条件优化[J].广西科学院学报,2026,42(2):143-155,13.基金项目
国家自然科学基金项目(32560023)和广西民族大学大学生创新创业训练计划项目(202410608026,202310608017,S202510608309)资助. （32560023）
