华侨大学学报（自然科学版）2026，Vol.47Issue(5)：562-570,9.DOI:10.11830/ISSN.1000-5013.202509046
西玛津降解菌的筛选与鉴定及其降解条件优化
Screening and Identification of Simazine-Degrading Bacteria and Optimization of Degradation Conditions
摘要
Abstract
To reduce the residue of simazine in the soil of vegetable planting areas in Xiamen City and improve the safety of agricultural products,the enrichment culture and plate streak method were used to screen a strain S-7 capable of degrading simazine.By observing morphological characteristics and determining the 16S rDNA sequence of the strain,the strain identification was carried out,and single-factor experiments and Box-Behnken response surface experiments were used to optimize the optimal conditions for strain S-7 to degrade simazine.The results showed that among the four strains screened and isolated from vegetable soil,strain S-7 exhibited the strongest simazine degradation ability,and it was a fungus belonging to the genus Candida after identified.The optimal degradation conditions for strain S-7 were temperature 29.7℃,pH value of 7.7,inoculation amount of 2.1%,simazine initial mass concentration of 40 mg·mL-1,glucose as the carbon source,and the degradation rate of 52.5%after 48 hours.Strain S-7 exhibited significant degradation ability towards sima-zine,and the optimized culture conditions could markedly enhance its degradation efficiency.关键词
西玛津/生物降解/Box-Behnken响应面法/热带假丝酵母菌Key words
simazine/biodegradation/Box-Behnken response surface method/Candida tropicalis分类
资源环境引用本文复制引用
李妍,于海玲,王明元,彭汇涵,詹玉琦,陈其煌,林永华..西玛津降解菌的筛选与鉴定及其降解条件优化[J].华侨大学学报（自然科学版）,2026,47(5):562-570,9.基金项目
福建省厦门市科技计划项目(3502Z20226030) （3502Z20226030）
