甘蔗糖业2026，Vol.55Issue(4)：18-25,8.DOI:10.3969/j.issn.1005-9695.2026.04.002
果蔗组培苗塑化标本制作工艺探究
Investigation on the Plastination Specimen Preparation Technology for Tissue-Cultured Seedlings of Chewing Cane
摘要
Abstract
To address the problems of tissue-cultured seedlings of chewing cane,such as susceptibility to contamination,high environmental requirements,and vulnerability to etiolation and wilting when used as living materials in popular science exhibitions and teaching research,this study employed tissue-cultured plantlets of black-skinned chewing cane as experimental materials to investigate the preparation process of their plastinated specimens.Four copper-salt green-preserving solutions were set up,corresponding to two preservation treatment methods:heating immersion and room-temperature immersion.After color preservation,two dehydration and plastination techniques,namely the polyethylene glycol(PEG)gradient method and the ethanol-PEG composite method,were applied for comparative experiments.The results showed that specimens treated with the PEG gradient method exhibited intact morphology,naturally stretched and flattened leaves,flexible texture,and maintained a vivid green and fresh appearance for an extended period,demonstrating significantly superior performance to the ethanol-PEG composite method.No significant differences in color-preserving efficacy were observed among the four green-preserving solutions under the PEG gradient method,allowing flexible selection between heating and room-temperature immersion protocols based on equipment availability and time cost considerations.The prepared specimens can be encapsulated in test tubes or tissue-culture bottles to simulate native culture scenarios,or embedded in epoxy resin AB adhesive for long-term preservation,offering both pedagogical practicality and popular-science ornamental value.The preparation process established in this study provides a stable and reliable physical medium for the achievement display of virus-free seedlings and the popular science dissemination of germplasm resources.关键词
果蔗/组培苗/塑化标本/铜盐保绿/脱水塑化/农业科普Key words
Chewing cane/Tissue-cultured plantlets/Plastinated specimens/Copper salt-based green preservation/Specimen dehydration and plastination/Agricultural science popularization分类
农业科技引用本文复制引用
何慧怡,陈勇生,曾姣,李淑华,陈迪文..果蔗组培苗塑化标本制作工艺探究[J].甘蔗糖业,2026,55(4):18-25,8.基金项目
广州市创新环境项目"果蔗品种改良及配套技术推广"(2025D04J0033) （2025D04J0033）
