河北中医药学报2026，Vol.41Issue(4)：64-68,80,6.
加减补阳还五汤调控AMPK/SIRT1/PGC-1α通路改善足细胞凋亡的机制研究
Mechanism of Modified Buyang Huanwu Decoction Improving Podocyte Apoptosis via Regulating AMPK/SIRT1/PGC-1α Pathway
摘要
Abstract
Objective:To investigate whether modified Buyang Huanwu Decoction improves high glucose-induced podocyte injury via activating the AMPK/SIRT1/PGC-1α signaling pathway.Methods:The medicated serum containing modified Buyang Huanwu Decoction was prepared,and the appropriate intervention concentration for cells was screened.Immortalized mouse podocytes(MPC5)were divided into control group,high glucose group and modified Buyang Huanwu Decoction group.The high glucose group was treated with high glucose stimulation,while the modified Buyang Huanwu Decoction group received medicated serum intervention simultaneously with high glucose stimulation.Cells were collected after intervention.Western blot was used to detect the expressions of Bcl-2,Cyt C,AMPKα,p-AMPKα,PGC-1α and SIRT1 in podocytes.Immunofluorescence assay was used to detect the levels of Mito-ROS and JC-1 in podocytes.Results:Compared with the control group,the high glucose group showed elevated fluorescence intensity of Mito-ROS and green fluorescence intensity of JC-1,as well as increased JC-1 fluorescence ratio(P<0.05).The expressions of Bcl-2,p-AMPKα,SIRT1 and PGC-1α were decreased,while the expressions of Bax and Cyt C were increased(P<0.05).After intervention with medicated serum of modified Buyang Huanwu Decoction,the fluorescence intensity of Mito-ROS and green fluorescence intensity of JC-1 in podocytes were reduced,and the JC-1 fluorescence ratio was decreased(P<0.05).Meanwhile,the expressions of Bcl-2,p-AMPKα,SIRT1 and PGC-1α were up-regulated,whereas the expressions of Bax and Cyt C were down-regulated(P<0.05).Conclusion:Modified Buyang Huanwu Decoction may inhibit high glucose-induced podocyte apoptosis by activating the AMPK/SIRT1/PGC-1α signaling pathway and improving mitochondrial function.关键词
糖尿病肾病/加减补阳还五汤/线粒体功能障碍/AMPK/SIRT1/PGC-1α通路/足细胞凋亡Key words
Diabetic nephropathy/modified Buyang Huanwu Decoction/mitochondrial dysfunction/AMPK/SIRT1/PGC-1α pathway/podocyte apoptosis分类
医药卫生引用本文复制引用
钟艳,杨帆,常奕,薛思思,熊云昭,张续默,张晓云..加减补阳还五汤调控AMPK/SIRT1/PGC-1α通路改善足细胞凋亡的机制研究[J].河北中医药学报,2026,41(4):64-68,80,6.基金项目
国家自然科学青年基金(82205006) （82205006）
河北自然科学基金(H2025423042,H2025423034) （H2025423042,H2025423034）
河北中医药管理局科技计划项目(2024102) （2024102）
河北中医药大学燕赵医学研究项目(YZSY2023003) （YZSY2023003）
河北中医药大学大学生创新创业训练计划项目(X202614432006) （X202614432006）
