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藜蒿废叶茎中挥发油提取工艺的响应面法优化及活性分析

王璐 徐媛 曾长立

江汉大学学报(自然科学版)2026,Vol.54Issue(4):5-14,10.
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江汉大学学报(自然科学版)2026,Vol.54Issue(4):5-14,10.DOI:10.16389/j.cnki.cn42-1737/n.2026.04.001

藜蒿废叶茎中挥发油提取工艺的响应面法优化及活性分析

Optimization of the Extraction Process of Essential Oil from Waste Leaves and Stems of Artemisia selengensis by Response Surface Methodology and Activity Analysis

王璐 1徐媛 1曾长立1

作者信息

  • 1. 江汉大学 生命科学学院,湖北省汉江流域特色生物资源保护开发与利用工程技术研究中心,湖北 武汉 430056
  • 折叠

摘要

Abstract

Artemisia selengensis is rich in nutrients,but only its fresh and tender young stems are edible.The discarded leaves and stems are prone to decay and deterioration,causing environmental pollution and hindering the development of a circular economy.In this study,essential oil was extracted from waste leaves and stems of A.selengensis by steam distillation.Based on single-factor experiments,the extraction yield of A.selengensis essential oil was used as evaluation index.Combined with Box-Behnken design(BBD)and response surface methodology(RSM),the optimal extraction conditions were determined as follows:extraction time of 5.5 h,solid-liquid ratio of 1∶18,and sodium chloride concentration of 20%,under which the maximum extraction yield reached 0.49%.The main components of the essential oil were analyzed by gas chromatography-mass spectrometry(GC-MS).The results indicated that the essential oil contained various compounds,including olefins,phenols,fatty acids,alcohols,ketones,and esters.Antibacterial assays showed that the essential oil exhibited inhibitory effects against Staphylococcus aureus,Escherichia coli,and Saccharomyces cerevisiae.The antioxidant results showed that the DPPH radical scavenging rate of the essential oil was(85.84±0.52)%,with a scavenging capacity of(148.43±1.29)μmol Trolox/100 g essential oil,while the ABTS radical scavenging rate was(16.34±0.43)%,with a scavenging capacity of(99.48±6.69)μmol Trolox/100 g essential oil,indicating that the essential oil possessed antioxidant activity.

关键词

藜蒿/挥发油/提取工艺/活性成分

Key words

Artemisia selengensis/essential oil/extraction process/bioactive components

分类

轻工纺织

引用本文复制引用

王璐,徐媛,曾长立..藜蒿废叶茎中挥发油提取工艺的响应面法优化及活性分析[J].江汉大学学报(自然科学版),2026,54(4):5-14,10.

基金项目

2020年度湖北省教育厅科学研究计划指导性项目(B2020223) (B2020223)

江汉大学学报(自然科学版)

1673-0143

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