江汉大学学报（自然科学版）2026，Vol.54Issue(4)：63-71,9.DOI:10.16389/j.cnki.cn42-1737/n.2026.04.006
肿瘤相关黏蛋白联合表达促胰腺癌作用研究
Study on the Promoting Effect of the Co-expression of Tumor-Associated Mucins on Pancreatic Cancer
摘要
Abstract
Objective To investigate,by bioinformatics methods,the co-expression of the tumor-associated mucin(MUC)genes MUC1,MUC4,MUC5B,and MUC13 and their roles in tumorigenesis,so as to provide new targets for the early diagnosis and immunotherapy of pancreatic adenocarcinoma(PAAD).Methods A pan-cancer analysis based on Timer 2.0 and TCGA+GTEx databases was performed to screen mucins highly expressed in PAAD.Protein expression differences were validated using the Proteomic Data Commons(PDC)database.Prognostic value was assessed via survival analysis and clinical stage association analysis.Wikipathways enrichment analysis was conducted using Gene Set Enrichment Analysis(GSEA).A nomogram model was constructed to quantify disease risk.Tumor mutation burden(TMB)of the mucins was analyzed.Protein expression was further validated by immunohistochemistry using the Human Protein Atlas(HPA)database.Results mRNA and protein expression levels of MUC1,MUC4,MUC5B,and MUC13 were significantly higher in PAAD tissues compared to adjacent normal tissues(P＜0.05).Overall survival(OS)was significantly prolonged in the low-mucin-expression group(P＜0.05).Mucin expression correlated with clinical stage:MUC1 was highest in stage Ⅳ,while MUC4,MUC5B,and MUC13 were highest in stage Ⅱ.GSEA revealed that the high-expression of MUC1,MUC4,and MUC5B significantly activated pancreatic tumor-associated signaling pathways,while the high-expression group of MUC13 significantly activated non-specific immune-related signaling pathways.The nomogram model indicated that when the combined mucin score reached 90 points,the risk of PAAD was as high as 80％.Mutations in the mucin genes were all significantly positively correlated with PAAD.Conclusion MUC1,MUC4,MUC5B,and MUC13 are synergistically overexpressed in PAAD and may participate in tumor progression and immune evasion by activating tumor-related pathways and promoting gene mutations.They may serve as biomarkers for the early diagnosis of PAAD and as potential targets for combined immunotherapy.关键词
胰腺癌/黏蛋白/生物信息学/肿瘤标志物/免疫检查点Key words
pancreatic cancer/mucins/bioinformatics/tumor biomarkers/immune checkpoints分类
医药卫生引用本文复制引用
杨光,邓雅轩,何琪,余子鸢..肿瘤相关黏蛋白联合表达促胰腺癌作用研究[J].江汉大学学报（自然科学版）,2026,54(4):63-71,9.基金项目
江汉大学校级学生科研项目(2024yb124) （2024yb124）
