耕作与栽培2026，Vol.46Issue(7)：24-28,5.
奉节烟区解钾功能菌的分离筛选及解钾能力研究
Isolation,Screening and Potassium-solubilizing Capacity of Potassium-solubilizing Bacteria from Fengjie Tobacco-growing Area
摘要
Abstract
To screen and isolate potassium-solubilizing functional strains from a tobacco-growing area and investigate their potassium-solubilizing capacity,soil samples were collected from the tobacco-planting region of Fengjie County,Chongq-ing Municipality.A total of 60 strains were isolated from the soil using the dilution spread-plate method combined with po-tassium feldspar selective medium.After primary and secondary screening,9 strains with relatively high potassium-solubilizing efficiency were obtained.Further liquid shake-flask fermentation combined with flame photometry for deter-mination of available potassium release led to the selection of two highly efficient potassium-solubilizing strains,desig-nated K2 and K6.At 20 ℃,strain K2 exhibited the strongest potassium-solubilizing capacity toward potassium feldspar,with available potassium release increased by 80%compared with the control.Strain K6 showed the most outstanding ca-pacity toward muscovite,with available potassium release increased by 133%compared with the control.Based on 16S rRNA gene sequence analysis and physiological and biochemical characterization,strain K2 was identified as Brevibacil-lus brevis,and strain K6 was identified as Paenibacillus mucilaginosus.This study provides excellent microbial resources and a theoretical basis for the development of potassium-solubilizing bacterial fertilizers specifically for tobacco cultiva-tion.关键词
奉节烟区/解钾功能菌/解钾能力/短短芽孢杆菌/胶质类芽孢杆菌Key words
Fengjie tobacco-growing area/potassium-solubilizing functional bacteria/potassium-solubilizing capacity/Brevibacillus brevis/Paenibacillus mucilaginosus分类
农业科技引用本文复制引用
罗宝雄,金亚波,沈方科..奉节烟区解钾功能菌的分离筛选及解钾能力研究[J].耕作与栽培,2026,46(7):24-28,5.基金项目
广西中烟工业有限责任公司项目(GXZYCX2017B007) （GXZYCX2017B007）
中国烟草总公司广西壮族自治区公司科技计划项目(202345120026059) （202345120026059）
