麦类作物学报2026，Vol.46Issue(8)：1049-1058,10.DOI:10.7606/j.issn.1009-1041.2026.08.06
小麦干热风胁迫下相关miRNA的筛选及其靶基因分析
Screening and Identification of miRNAs Related to Dry-Hot Wind Stress in Wheat and Analysis of Their Target Genes
摘要
Abstract
To elucidate the molecular mechanism of miRNA in wheat's response to dry-hot wind stress,a sensitive germplasm Xinchun 14 and a tolerant variety Xinchun 46 were selected as materials.Dur-ing the grain-filling stage,artificial simulation of dry-hot wind stress(daytime temperature 35 ℃/nighttime temperature 25 ℃,relative humidity 20％,wind speed 5 m·s-1,continuous treatment for 5 days)was applied.High-throughput sequencing technology was used to screen differentially ex-pressed miRNAs,followed by validation via qRT-PCR.The results showed that 203 miRNAs were involved in the expression under dry-hot wind stress,with 136 co-expressed miRNAs.After differen-tial screening,six core differentially expressed miRNAs were ultimately identified,namely,novel_miR_537,novel_miR_443,novel_miR_469,novel_miR_393,novel_miR_390,and novel_miR_339.These miRNAs were significantly enriched in four pathways:the biosynthesis of mannose O-glycans,the degradation of polysaccharides,peroxisomes,and the metabolism of selenium compounds.Target gene predictions revealed that these miRNAs targeted and regulated key membrane protein-encoding genes,including β-1,2-xyltransferase,α-1,3-arabinosyltransferase,GDSL esterase/lipase,α-L-fucosi-dase,and peroxidase,and are involved in the maintenance of cell wall stability,reactive oxygen spe-cies clearance,and osmotic regulation processes.This indicates there is a miRNA regulatory network in the response of dry-hot wind in wheat,and it jointly participates in the resistance process of wheat to dry-hot wind stress in the form of a'miRNA-targeted gene'molecular module.关键词
小麦/干热风胁迫/miRNA/靶基因/分子调控/抗逆育种Key words
Triticum aestivum/Hot-dry wind stress/miRNA/Target genes/Molecular regulation/Stress-resistant breeding分类
农业科技引用本文复制引用
高新,雷嫣,苏豫梅,张宏芝,李少杰,李剑峰,申有成,王重,张跃强..小麦干热风胁迫下相关miRNA的筛选及其靶基因分析[J].麦类作物学报,2026,46(8):1049-1058,10.基金项目
新疆自治区自然科学基金(青年基金)项目(2022D01B159) （青年基金）
中央引导地方科技发展专项(ZYYD2024CG04) （ZYYD2024CG04）
中央引导地方科技发展专项(ZYYD2024JD18) （ZYYD2024JD18）
新疆自治区重点研发专项(2022B02001-3) （2022B02001-3）
新疆现代农业小麦产业技术体系项目(XJARS-01) （XJARS-01）
新疆维吾尔自治区2024年度大学生创新训练计划项目(dxscx2024474) （dxscx2024474）
