口腔颌面外科杂志2026，Vol.36Issue(4)：281-289,9.DOI:10.12439/kqhm.1005-4979.2026.04.003
巨噬细胞Trem2对牙周炎小鼠拔牙后牙槽窝骨愈合影响的实验研究
The effect of macrophage Trem2 on alveolar socket bone healing after tooth extraction in mice with periodontitis:In vitro and in vivo experiments
摘要
Abstract
Objective:To explore the impact of triggering receptor expressed on myeloid cells 2(Trem2)on alveolar socket bone healing in mice with periodontitis after tooth extraction.Methods:A murine periodontitis model was established using the silk ligature method,and changes in the expression of TREM2⁺ macrophages within the alveolar socket were detected by immunofluorescence staining.A periodontitis tooth-extraction model was established using Trem2 gene knockout(Trem2-/-)mice,and alveolar socket bone healing was evaluated by Micro-CT,Masson staining,and tartrate-resistant acid phosphatase(TRAP)staining.Mouse bone marrow-derived macrophages(BMDMs)and bone marrow mesenchymal stromal cells(BMSCs)were cultured in vitro to establish an indirect co-culture system,and the osteogenic differentiation capacity of BMSCs was assessed by real-time quantitative polymerase chain reaction(RT-qPCR)and alkaline phosphatase(ALP)staining.Results:The number of TREM2⁺ macrophages within the alveolar socket increased after tooth extraction in mice with periodontitis.Compared with wild-type(WT)mice,Trem2-/-mice exhibited reduced new bone formation,a decreased bone volume fraction(bone volume/tissue volume,BV/TV),and decreased new bone collagen deposition within the alveolar socket at 7 and 14 days after tooth extraction;the number of osteoclasts within the alveolar socket increased at 14 days after tooth extraction.In vitro experiments showed that,compared with the conditioned medium from the WT group,the conditioned medium from the Trem2-/-group induced lower expression levels of Alp,Runx2,and Sp7 and weaker ALP activity in BMSCs.Conclusion:In the microenvironment of periodontitis,macrophage Trem2 plays a crucial regulatory role in alveolar socket healing.In the in vitro indirect co-culture system with BMSCs,reduced Trem2 expression attenuates the ability of BMDMs to induce the osteogenic differentiation of BMSCs.关键词
牙周炎/牙拔除术/巨噬细胞/髓样细胞触发受体2Key words
periodontitis/tooth extraction/macrophage/triggering receptor expressed on myeloid cells 2分类
医药卫生引用本文复制引用
何雅琳,翁雨藤,王佐林..巨噬细胞Trem2对牙周炎小鼠拔牙后牙槽窝骨愈合影响的实验研究[J].口腔颌面外科杂志,2026,36(4):281-289,9.基金项目
国家自然科学基金(82201033) （82201033）
国家重点研发计划(2018YFE0202200) （2018YFE0202200）
