口腔颌面外科杂志2026，Vol.36Issue(4)：290-298,9.DOI:10.12439/kqhm.1005-4979.2026.04.004
载氨基胍的铜介孔二氧化硅对MGO刺激下大鼠BMSCs成骨分化的影响
Effect of copper-composite mesoporous silica nanoparticles loaded with aminoguanidine on osteogenic differentiation of MGO-stimulated rat BMSCs
摘要
Abstract
Objective:To synthesize copper-composite mesoporous silica nanoparticles loaded with aminoguanidine(AG@Cu-MSNs)and investigate their effects on the osteogenic differentiation of methylglyoxal(MGO)-stimulated rat maxillary bone marrow stromal cells(M-BMSCs).Methods:Copper-composite mesoporous silica nanoparticles(Cu-MSNs)were prepared by the sol-gel method,and AG@Cu-MSNs were prepared by dispersing Cu-MSNs in deionized water containing aminoguanidine.The nanoparticles were characterized by scanning electron microscopy and transmission electron microscopy,and other techniques.M-BMSCs were cultured.The CCK-8 assay kit was used to determine the optimal stimulatory concentration of MGO on M-BMSCs and the co-culture concentration of composite nanoparticles with M-BMSCs.The live/dead staining assay was employed to assess the impact of composite nanoparticles on cell viability in MGO-stimulated conditions.After 7 days of mineralization induction,alkaline phosphatase(ALP)activity was assessed using an ALP assay kit.Real-time quantitative polymerase chain reaction(RT-qPCR)was used to detect the expression of some osteogenic related gene.Western blotting was used to detect the expression of osteogenesis-related proteins.Results:The application concentration of MGO was determined to be 0.5 mmol/L by CCK-8 assay.The results of CCK-8 assay and live/dead staining assay showed that the composite nanoparticles had good biocompatibility.Among them,the AG@Cu-MSNs group exhibited the highest cell viability and proliferation in MGO-stimulated conditions.After 7 days of mineralization induction,compared with the MGO stimulation group,the AG@Cu-MSNs group exhibited the most pronounced ALP staining intensity.Additionally,the expression levels of osteogenesis-related genes(Alpl,Runx2,Osx)and corresponding proteins(RUNX2,OSX)were significantly upregulated.Conclusion:In MGO-stimulated conditions,AG@Cu-MSNs significantly promotes the proliferation and osteogenic differentiation of M-BMSCs.关键词
甲基乙二醛/纳米颗粒/氨基胍/铜离子/成骨分化Key words
methylglyoxal/nanoparticles/aminoguanidine/copper ions/osteogenic differentiation分类
医药卫生引用本文复制引用
保静,胡丝桐,苏俭生..载氨基胍的铜介孔二氧化硅对MGO刺激下大鼠BMSCs成骨分化的影响[J].口腔颌面外科杂志,2026,36(4):290-298,9.基金项目
上海市科学技术委员会项目(18441902100) （18441902100）
