麦类作物学报2026，Vol.46Issue(8)：1082-1092,11.DOI:10.7606/j.issn.1009-1041.2026.08.09
盐胁迫下青稞幼苗生理响应和转录组分析
Physiological Response and Transcriptome Analysis of Highland Barley Seedlings under Salt Stress
摘要
Abstract
In order to explore the physiological and molecular response mechanism of highland barley seedlings to salt stress,ZYM1795,ZYM3130 and ZYM2239 highland barley seedlings were used as materials.The seedlings were treated with 50,150,and 250 mmol·L-1 NaCl solution and distilled water(control).The contents of malondialdehyde(MDA),total soluble sugar(SS)and the activities of catalase(CAT),superoxide dismutase(SOD),and peroxidase(POD)in the leaves of highland bar-ley were determined.Transcriptome sequencing was performed on the leaves of the control and 250 mmol·L-1 NaCl treatment.The results showed that with the increase of salt concentration,the MDA content of salt-tolerant materials increased first and then decreased;the activities of SOD,POD and CAT increased first and then decreased;and the SS content accumulated significantly,indicating that highland barley responded to salt stress by activating antioxidant defense system and osmotic ad-justment substances.Through transcriptome analysis,a total of 3 343 differentially expressed genes that may be related to salt tolerance were identified.Through weighted gene co-expression network analysis,yellow modules significantly related to all physiological indicators were screened,and 1 801 potential salt tolerance-related genes were further obtained.GO enrichment analysis showed that these genes were mainly enriched in stress response,antioxidant defense,hormone signal transduc-tion,such as abscisic acid response,enzyme activity regulation and other related pathways.Among them,20 key genes were screened,including peroxidase gene,trehalose phosphorylase gene,serine/threonine protein kinase gene,and ABC transporter gene.These genes play an important role in ac-tive oxygen scavenging,osmotic regulation,and signal transduction.It is speculated that highland barley seedlings enhance salt tolerance by synergistically regulating physiological response and key gene expression.关键词
青稞幼苗/盐胁迫/生理指标/转录组/加权基因共表达网络分析Key words
Highland barley seedlings/Salt stress/Physiologic index/Transcriptome/Weighted gene co-expression network analysis分类
农业科技引用本文复制引用
莫洪婷,黄新荣..盐胁迫下青稞幼苗生理响应和转录组分析[J].麦类作物学报,2026,46(8):1082-1092,11.基金项目
国家复份库运行服务科技部平台项目(NCGRC-2024-20) （NCGRC-2024-20）
青海大学青年科研基金项目(2023-QNY-8) （2023-QNY-8）
青海大学大学生科研训练计划项目(SRT202570) （SRT202570）
