林业科学研究2026，Vol.39Issue(4)：170-176,7.DOI:10.12403/j.1001-1498.20250314
4个云杉树种胚性愈伤组织的诱导研究
Induction and Histological Characterization of Embryogenic Callus in Four Spruce Species
摘要
Abstract
[Objective]This study aimed to compare the efficiency of embryogenic callus among four spruce species,namely Picea species,Picea abies,P.likiangensis,P.glauca,and P.mariana,and to provide a basis for screening efficient induction media suitable for different species and genotypes.[Methods]Based on the cones from five seed lots representing the four species,cone size was measured,and the developmental stages of zygotic embryos were observed.Embryogenic callus induction trials were con-ducted,and histochemical staining was subsequently performed to characterize the structure of induced callus.[Results]Cone size differed significantly among the four species,following the order:P.abies＞P.likiangensis＞P.glauca＞P.mariana.Zygotic embryo development varied markedly both among species and among individual trees within the same species,ranging from the early embryonic stage to the mature embryonic stage.Analysis of induction rates revealed that P.likiangensis exhibited the highest interspecif-ic induction rate(20.16％).Among the different seed lots,the first lot of P.abies and the fifth lots of P.liki-angensis and P.glauca showed the highest induction rates.Histochemical staining further confirmed that calli induced from all four species exhibited typical embryogenic characteristics,including densely ar-ranged,highly active cells in the embryonal head region and extensively vacuolated cells in the suspensor region.[Conclusion]The four tested Picea species exhibited significant differences in cone size,with P.abies producing the largest cones.P.likiangensis demonstrated the highest induction efficiency,while all four species successfully produced callus with definitive embryogenic structures.These results provide a useful reference for optimizing embryogenic callus protocols across diverse Picea species.关键词
云杉属/合子胚/胚性愈伤组织/诱导率/组织化学染色Key words
Picea/zygotic embryo/embryogenic callus/induction rate/histochemical staining分类
农业科技引用本文复制引用
骞树梅,胡继文,朱天擎,安三平,王丽芳,王军辉,许洋..4个云杉树种胚性愈伤组织的诱导研究[J].林业科学研究,2026,39(4):170-176,7.基金项目
国家重点研发计划(2023YFD2200603) （2023YFD2200603）
中央引导地方科技发展资金项目(24ZYQE004) （24ZYQE004）
甘肃省重点研发计划项目(25YFNE001). （25YFNE001）
