实验流体力学2026，Vol.40Issue(4)：前插1-前插2,1-21,23.DOI:10.11729/syltlx20250127
面向单细胞封装的微流控技术及其流动机理综述
A review on techniques and their flow mechanisms in microfluidics for single-cell encapsulation
摘要
Abstract
Precise cellular analysis is one of the core goals in contemporary life science research.As a key prerequisite for achieving this goal,single-cell encapsulation effectively isolates individual cells from complex cell populations.Microfluidic technology,by precisely manipulating microscale flow dynamics,provides robust technical support for efficient and controllable single-cell isolation or encapsulation.From the perspective of microscale flow,this work systematically reviews microfluidic technologies for single-cell encapsulation,focusing on flow mechanisms,technological evolution,and performance regulation strategies for encapsulation methods and for enhancing encapsulation efficiency.Single-cell encapsulation achieves cell capture and encapsulation through hydrodynamic effects such as low-Reynolds-number flow and interface instability,while encapsulation enhancement technologies overcome the limitations of conventional encapsulation efficiency by regulating processes like cell separation and focusing,thereby enabling efficient cell encapsulation.Understanding flow mechanisms helps improve encapsulation performance.By elucidating flow mechanisms to enhance encapsulation efficacy,this study collates hydrodynamic distinctions across methodologies,articulates their cardinal advantages and inherent limitations,furnishes theoretical guidance for mechanistic research and experimental design in single-cell encapsulation,and fosters deeper integration of microfluidic approaches within biofluid mechanics.关键词
微流控技术/单细胞封装/界面不稳定/流动聚焦/惯性聚焦Key words
microfluidic technology/single-cell encapsulation/interface instability/flow focusing/inertial focusing分类
数理科学引用本文复制引用
刘安迪,胡国辉..面向单细胞封装的微流控技术及其流动机理综述[J].实验流体力学,2026,40(4):前插1-前插2,1-21,23.基金项目
国家自然科学基金项目(12332016,12402323) （12332016,12402323）
